Thermo Scientific Dionex ICS-5000, Operator'S Manual

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Dionex ICS-5000 Operator’s Manual
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Doc. 065342-06 12/11
time (in minutes) is on the x-axis and the detector response (in
nanoCoulombs) is on the y-axis.
For 3D amperometry data, Chromeleon stores raw data points at a rate of
1 KHz throughout the entire run. Each raw 3D data point is defined by the
following attributes: the detector current (1) recorded at a particular
waveform time (2) and at a specific retention time (3). As a result, the two-
dimensional view of an integrated amperometry chromatogram (retention
time vs. integrated current) is extended by a third dimension (waveform time).
Thus, data for the entire waveform period—not just the integration
intervals—are collected.
Displaying and Reprocessing 3D Data
This section describes how to display and reprocess 3D amperometry data
using Chromeleon 6.8. If you are using Chromeleon 7, the features described
here are available by displaying the data in a contour plot. For details, refer to
the Chromeleon 7 Help.
In Chromeleon 6.8 you can display and reprocess 3D amperometry data in the
3D Amperometry View window (see
Figure 2-57 ). To open the window,
Figure 2-56. Example Chromatogram for a Pulsed Amperometry Application
0
1
2
3
4
5
6
Minutes
0 5 10
40
Analytical Column: CarboPac PA10
and
Guard
Eluent: 18 mM NaOH
Flow Rate: 1.5 mL/min
Detection: Pulsed Amperometry
Electrode: Gold
Waveform: Quadruple Potential
(4 x 250 mm)
Peaks: 1. L-Fucose
2. D-Galactosamine
3. D-Glucosamine
4. D-Galactose
5. D-Glucose
6. D-Mannose
Retention Time (Minutes)
Response
(nC)