On a clean and level surface:
1.
Place the tubes in the MicroAmp
™
96-well tray, then place the tray retainer over
the tubes.
2.
Prepare the sample according to your application protocol, then pipet the sample
into the tubes.
3.
Place a septum on the tubes.
a.
Align the holes of the septa with
the tubes.
b.
Press gently until the septum
are inserted into position in
each tube.
4.
Centrifuge the tube assembly briefly
to collect the contents at the bottom
of each tube.
Centrifuge the tube assembly again
if the contents are not at the bottom
of the tubes.
5.
Heat-denature as required.
6.
Place the tube assembly on ice for 3 minutes.
Load the tube assembly onto the instrument immediately or keep the tubes on ice and
protected from light until they are loaded onto the instrument.
Prepare the instrument
1.
Touch Settings
4
Consumable status.
2.
Ensure that:
• Sufficient consumables are installed for the run.
• Installed consumables have not exceeded their expiry date.
Prepare the tubes
1
2
3
4
1
Septum
2
96-well retainer
3
8-tube strip
4
MicroAmp
™
96-well tray
Check the
consumables
status
Chapter 2
Prepare the samples and the instrument
Prepare the instrument
2
SeqStudio
™
Genetic Analyzer Instrument and Software User Guide
39
