Condition number
A Condition Number indicates the amount of overlap between the dye peaks in the
fluorescence emission spectra of the dyes in the dye set.
If there is no overlap in a dye set, the Condition Number is 1.0 (ideal conditions), the
lowest possible value. The condition number increases with increasing peak overlap.
The ranges that the software uses to determine if a capillary passes or fails are:
Dye Set
Quality Value Minimum
Condition Number
Maximum
AnyDye
0.8 (default)
20.0 (default)
D
0.8
8.5
E
0.95
5.5
E5
0.95
6.0
F
0.95
8.5
G5
0.95
13.5
J6
0.95
8.0
J6-T
0.95
8.0
Z
0.95
5.5
Add a custom dye set to the software based on a system dye
1.
In the home screen, touch Settings
4
Maintenance and Service
4
Calibration
4
Dye calibration.
2.
Touch Custom Dye.
3.
Touch Add.
Perform a custom
dye calibration
Appendix C
Dye calibration and install standard checks
Calibrate dyes
C
SeqStudio
™
Genetic Analyzer Instrument and Software User Guide
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