View the raw data and the EPT plot
The EPT view (ElectroPhoresis Telemetry) shows instrument data conditions
(currents, temperatures, electrophoresis voltage) as a function of time.
When a run is complete, in the home screen:
1.
Touch Results.
2.
Touch List view.
3.
Touch an injection group.
4.
Touch a sample file name.
If the data triggered any quality alerts, a QC alerts screen is displayed.
Click View data to display the trace for the sample.
5.
Touch or to scroll to the raw data or EPT Plot.
Data quality alerts
Quality alert
Description
Action
Offscale peaks. Adjust the injection
parameters and/or the sample
concentration.
At least 10 scans have saturated the
CCD camera.
• Reduce the injection voltage or
time.
• Dilute the sample.
No sample was detected.
Poor signal
‑
to
‑
noise ratio with low
signal detected.
• Verify that the sample volume
follows recommendations in the
user manual.
• Troubleshoot upstream PCR and
sequencing steps.
Sizecalling and basecalling quality alerts
Table 13
Sizecalling quality alerts
Quality alert
Description
Action
Sizing quality value is low due to poor size
standard peak quality. Peak height uniformity is
low or the fitting quality in sizing is poor.
Low resolution or poor quality
data is present.
• Re-inject the sample.
• If the problem persists,
check the sample quality.
Sizecaller found broad peak(s) in the size
standard peak(s).
Sizing quality value is in the intermediate range;
check size standard data quality.
Appendix A
Troubleshooting
View the raw data and the EPT plot
A
SeqStudio
™
Genetic Analyzer Instrument and Software User Guide
171
