Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer, User Manual

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b.
Enter a name and edit settings as needed (see “Fragment/HID analysis
settings (size calling)“ on page 126).
c.
As needed, touch View or Delete (user-created settings only).
3. Touch Done.
Edit sequencing settings
Factory-installed items cannot be edited or deleted. To create a new item from a
factory-installed item, copy, edit, then save the new item.
Analysis settings are accessible only to the user who creates them.
1.
Access the Analysis settings screen:
From Action
Plate properties tab Touch More options
4
Analysis settings, then select the
settings to assign to the plate setup.
Home screen Touch Settings
4
Run settings
4
Analysis settings, then
manage the list of settings that you can select from during
plate set up.
2. To create new analysis settings:
a.
Touch the default analysis settings or user-created analysis settings, then
touch Copy.
b.
Enter a name and edit settings as needed (see “Sequencing settings (base
calling)“ on page 129).
c.
As needed, touch View or Delete (user-created settings only).
3.
Touch Done.
Fragment/HID analysis settings (size calling)
Setting
Description
Size calling method
• Local Southern— (default) Determines the fragment sizes using the reciprocal
relationship between fragment length and electrophoretic mobility.
• Global Southern—Compensates for standard fragments with anomalous
electrophoretic mobility (similar to least squares methods).
• 2nd LSQ (2nd Order Least Squares)—Uses regression analysis to build a bestfit
size calling curve.
• 3rd LSQ (3rd Order Least Squares)—Uses regression analysis to build a bestfit
size calling curve.
• Cubic Spline Interpolation—Forces the sizing curve through all the known points
of the selected size standard.
Chapter 10 Manage the software (instrument)
Manage run settings (instrument)
10
SeqStudio
™
Genetic Analyzer Instrument and Software User Guide 143