Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer, User Manual

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Run module
Contiguous read
length (CRL)
[1]
QV threshold
Approximate run
time
LongSeq
≥
800 QV20 ~ 2 hours
LongSeq_BDX
[1]
CRL was determined using the Long Read Sequencing standard. A minimum of 90% of analyzed sequences with an average QV ≥QV threshold
were observed.
IMPORTANT!
Use BDX run modules only if you prepare samples with BigDye
XTerminator
™
Purification Kit. Use non-BDX run modules for samples purified with
other methods.
Table 8 Fragment analysis run modules
Run module
Resolution
range
Approximate
run time
Sizing precision
Compatible size
standards
SNaPshot 40–120 bp 25 minutes 40–120: <0.5 GeneScan ™ 120 LIZ ™ Size
Standard
FragAnalysis 60–460 bp
[1]
45 minutes 60–460: <0.15 All except GeneScan ™
1200 LIZ ™ Size Standard
LongFragAnalysis
[2]
60–600 bp
[1]
< 2 hours 60–460: <0.15
461–600: <0.3
601–800: >0.45
• GeneScan ™ 600 LIZ ™
Size Standard v2.0
• GeneScan ™ 1200
LIZ ™ Size Standard
[1]
Resolution Range: The range of bases over which the resolution (peak spacing interval divided by the peak width at half-max in a GS600 or
GS1200 LIZ size standard sample sized with a third order fit) is
≥
1. The table shows the resolution range in
≥
90% of samples.
[2]
Load a maximum of 48 samples per plate if you use a long run module.
Table 9 HID run modules
Run module
Resolution
range
Approximate
run time
Sizing precision Compatible size standards
HIDAnalysis 60–470 bp
[1]
39 minutes 60–470: <0.15
• GeneScan
™
500 LIZ
™
Size
Standard
[2]
• GeneScan ™ 600 LIZ ™ Size
Standard v2.0
[1]
Resolution Range: The range of bases over which the resolution (peak spacing interval divided by the peak width at half-max in a GS600 size
standard sample sized with a third order fit) is
≥
1.
[2]
The GeneScan ™ 500 LIZ ™ Size Standard was not included in the HID validation of the instrument.
Note: The following size standards have not been validated for use with the
instrument. A default size standard definition is not provided in the software.
·
GeneScan
™
350 ROX
™
Size Standard
·
GeneScan
™
400HD ROX
™
dye Size Standard
Chapter 10 Manage the software (instrument)
Manage run settings (instrument)
10
SeqStudio
™
Genetic Analyzer Instrument and Software User Guide 141