Sequence analysis trace
7
4
1
3
5
6
2
1
Quality Value bars and values:
• Pure base with QV
³
20
• Pure base with QV 15–19
• Pure base with QV <15
• Mixed base
2
Bases—Mixed bases are highlighted in red. You can set the secondary peak threshold, as a
percentage of the primary peak, for consideration as a mixed base by the basecalling
algorithm. Reaching this threshold is a necessary but not sufficient condition for arriving
at a mixed base determination. See “Sequencing settings (base calling)“ on page 129.
3
Trace color hide/show—Touch to open, then touch a color to hide or show.
4
Analyzed trace
5
Thumbnail trace—Drag the center of the pane in the thumbnail trace to display another
trace area in the top pane. Drag the right or left handle of the pane to zoom horizontally.
6
Zoom tools—Touch to open.
7
Next trace tool—Touch to view the raw trace or EPT for the well.
Sequencing results
Result
Description
Quality
QC flags
•
—All QC tests passed.
•
—At least 1 warning quality alert was triggered.
•
—At least 1 failing quality alert was triggered.
Sequence analysis
results
Chapter 8
View and analyze results
View results on the instrument
8
SeqStudio
™
Genetic Analyzer Instrument and Software User Guide
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