tecan sunrise 6F Technical Manual Download Page 1

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TECAN

Technical Manual for

sunrise

RC, TW, TS, TC, BC, 6F

Document Part No. T 137 302

2004-10

Document Revision: 1.1

Firmware Revision Level: 3.xx

Summary of Contents for sunrise 6F

Page 1: ...TECAN Technical Manual for sunrise RC TW TS TC BC 6F Document Part No T 137 302 2004 10 Document Revision 1 1 Firmware Revision Level 3 xx...

Page 2: ...0 Fax 49 79 51 50 38 Italy Tecan Italia S r l Via F lli Cervi Palazzo Bernini Centro Direzionale Milano2 20090 Segrate Mi Italy Tel 39 02 215 21 28 Fax 39 02 215 97 441 Japan Tecan Japan Co Ltd Meiji...

Page 3: ...2 Gr dig Salzburg AUSTRIA EUROPE Telephone 0043 0 6246 8933 FAX 0043 0 6246 72770 Email office austria tecan com Copyright Information The contents of this document are the property of Tecan Austria G...

Page 4: ...s used in this publication appear below Note Gives helpful information Caution Indicates a possibility of instrument damage or data loss if instructions are not followed WARNING INDICATES THE POSSIBIL...

Page 5: ...04 10 Technical Manual for sunrise RC TW TS TC BC 6F No T 137 302 Rev No 1 1 5 Symbols Manufactured by Date of manufacture Conformit Europ enne In vitro diagnostic medical device Laser Biohazardous IV...

Page 6: ...t Europ enne cm Centimeter F Fahrenheit Hz Hertz IVD In vitro diagnostics IVD D In vitro diagnostics Directive kg Kilogram l L Liter LED Light emitting diode MB Megabyte l Microliter ml Milliliter nm...

Page 7: ...nt 6 Filter Carriage 2 9 2 3 6 Temperature Control 2 10 2 3 7 Barcode Laser Scanner 2 10 2 3 8 Microplates 2 11 2 3 9 USB Stick 2 12 2 4 Instrument Description 2 12 2 4 1 Back Panel Connections 2 13 2...

Page 8: ...4 9 4 4 4 Device configuration 4 10 4 4 5 Mirror and lamp adjustment 4 10 4 4 6 Plate calibration 4 11 4 4 7 Measurement offset Inhouse plate is necessary 4 12 4 4 8 User Configuration 4 13 4 4 9 Sun...

Page 9: ...onic System 8 1 Introduction 8 1 8 2 Interconnection Diagram Standard RC 8 2 8 3 Voltage Diagram Standard RC 8 3 8 4 Main Board 8 4 8 5 Replacing the Main Board 8 5 8 6 Power Board Standard 8 7 8 7 Re...

Page 10: ...Procedure 10 2 10 3 Sunrise SUNSET Software 10 2 10 4 QC Pac 2 Service Software 10 3 10 5 Flashcard Download for Touchscreen Unit 10 4 10 6 Barcode Upgrade Kit 10 5 10 6 1 General 10 5 10 6 2 Install...

Page 11: ...4 Disposal of the Instrument 11 12 12 Performance Testing Quality Control 12 1 Introduction 12 1 12 2 Optimizing for Maximum Performance 12 1 12 2 1 Instrument Location 12 1 12 2 2 Operating Procedur...

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Page 13: ...ult in damage to the product injury to operating personnel or poor instrument performance 3 Observe all WARNING and CAUTION statements in this publication 4 Never open sunrise while the instrument is...

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Page 15: ...manipulations in connection with samples or reagents must be strictly followed Taking this fact into consideration results must be interpreted carefully By reading twelve wells simultaneously the inst...

Page 16: ...directive A range of policies and standard operating procedures according to local regulations must also be established Make sure that the instrument and the software are suitable for use with the rea...

Page 17: ...research use for food analysis and veterinary applications The sunrise Absorbance Reader has been validated for use with representative applications from the above list The validation shows the gener...

Page 18: ...ions work with all hardware variations and accessories and can be used in any combination sunrise Options Temperature Control Touchscreen Barcode sunrise Accessories The sunrise accessories work with...

Page 19: ...eds Options such as a Touchscreen combined with a WindowsCE based on board software free wavelength selection temperature control and a barcode scanner can be added onto the basic system which is dedi...

Page 20: ...de approx 50 VA Operational mode max 110 VA Fuse rating 2 x F 2 0 A 250 V Fast Blow Outside dimensions Width 33 5 cm Depth 32 0 cm Height 17 0 cm Weight 6 kg Ambient temperature Operation 15 C to 35 C...

Page 21: ...etter than 1 0 0 010 Abs Better than 1 5 0 010 Abs Precision 492 nm 0 000 2 000 Abs 492 nm 2 000 3 000 Abs Better than 0 5 0 005 Abs Better than 1 0 0 005 Abs Linearity 340 399 nm 0 000 2 000 Abs 400...

Page 22: ...erating software MagellanCE Memory 64 MB RAM Storage 32 MB USB stick Computer interface Serial RS 232 C Printer USB master USB stick USB master Printer USB slave ACS MagellanCE software 300 38 400 bau...

Page 23: ...bs Resolution 0 001 Abs Accuracy 492 nm 0 000 2 000 Abs Better than 1 5 0 010 Abs Precision 492 nm 0 000 2 500 Abs Better than 1 0 0 005 Abs Linearity 492 nm 0 000 2 500 Abs Better than 2 Wavelength s...

Page 24: ...received in the instrument The microplate must not be the same as the one used for measurement but should be clean to keep influences on the following measurement low Every time the plate transport is...

Page 25: ...overall plate height 14 35 mm 0 76 mm 0 5650 inches 0 0299 inches Diameter of wells 7 0 mm 0 276 inches Pitch size center to center 9 0 mm 0 3543 inches Bottom shape V shaped Flat and Round bottom Th...

Page 26: ...ntion Up to 10 years Shock resistance 1000 G Max Erase cycles 1 000 000 times Speed Read 8 0 MB s Write 7 0 MB s Do not expose the USB stick to extreme temperatures fire water moisture dust smoke elec...

Page 27: ...60950 UL 1950 or CSA C22 2 No 950 for Data Processing Devices Barcode Laser Scanner Labels Label reproductions on the instrument housing and interior Note If any labels become damaged or removed conta...

Page 28: ...the plate support is fully ejected as illustrated below and the plate transport motor is not active For details see the corresponding Instructions for Use of the selected SW product Magellan Magellan...

Page 29: ...filter carriage has up to four narrow band interference filters that have a fixed wavelength SUNRISE Standard Filter Carriage When a wavelength is selected the entered wavelength is compared against t...

Page 30: ...e gradient filter carriages are calibrated by the manufacturer and each one is unique Note When another gradient filter is inserted into the instrument the instrument must be re calibrated This re cal...

Page 31: ...with the sunrise 6 Filter and the tuneable wavelength Absorbance Reader 2 6 Instrument Features Microplates can be measured using the following features Various measurement modes Single or dual wavele...

Page 32: ...al density is measured at three positions If an agglutination measurement is performed up to 40 measurement positions per well are used Note For more information about setting the measurement mode see...

Page 33: ...nce Reader Part of the Software CD Software Functions Magellan Instrument control and data reduction software IVD version available which has been declared suitable for in vitro diagnosis IVD purposes...

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Page 35: ...olve unpacking environmental requirements power requirements and interfacing 3 2 Unpacking and Inspection The delivered instrument is shipped in one carton which includes Power cable Computer connecti...

Page 36: ...roken parts Report any damage immediately 5 Compare the instrument s serial number attached on the rear panel of the instrument against the serial number of the instrument on the delivery shipping not...

Page 37: ...irements The instrument should be placed on a flat level surface that is free from dust solvents and acidic vapors Vibration and direct sunlight must be avoided to ensure correct results Ambient Tempe...

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Page 39: ...led using the following procedure Insert TECAN Detection Suite CD into the required CD ROM drive Double click the Setup icon under Software RdrDownload to start the installation program A series of di...

Page 40: ...ose the program or disconnect the instrument from the program To start the Firmware Download Software click the RdrDownload icon on the desktop or click the Start button on the lower taskbar and selec...

Page 41: ...select the correct upd file from the new window See picture below Click Download and the new firmware is Downloaded Note Do NOT switch off the instrument during the Download process When the Download...

Page 42: ...bles the user to define the settings of Instrument modes Filter definition Measurement modes 4 3 1 Installation of sunrise Instrument Settings Software The sunrise Instrument Settings software is inst...

Page 43: ...art button on the lower taskbar and select Programs TECAN and select sunrise Instrument Settings The following dialog box is displayed Select the correct communication port and the baudrate Click Next...

Page 44: ...lter slide In button and the filter is inserted Pos1 4 show the filter values for the currently loaded absorbance filters Note The instrument is able to recognize predefined filter slides and the filt...

Page 45: ...4 3 5 Define Measurement Mode The following dialog box is displayed Select the appropriate measurement mode Click Finish and the following dialog box is displayed The measurement mode has now been set...

Page 46: ...ext to continue The files are then installed and the program icon is created When the Installation Complete dialog box appears click Finish and SunSet Software is operational 4 4 2 Starting the SunSet...

Page 47: ...k OK The SunSet Software shows the connection to the reader The reader type Serial No date and time are displayed 4 4 3 Setup All Select Setup all from the Setup menu to adjust the instrument All of t...

Page 48: ...following dialog box is displayed Select the first filter and click Start The dialog box shows the minimum and maximum values in percent Adjust the three screws until the minimum and the maximum perc...

Page 49: ...s not possible to get better results the fiber optic must be replaced See chapter 6 9 Replacing Fiber Bundle 4 4 6 Plate calibration Select Plate calibration from the Setup menu The following dialog b...

Page 50: ...justment The plate carrier moves out and the following dialog box is displayed Insert the Inhouse plate with the A1 positioned top left and click OK The instrument starts the measurement and adjusts a...

Page 51: ...perly When using the Tuneable Wavelength option you must enter filter table values in the Gradient filter text boxes for each wavelength and you must also enter the Filter slide ID number of the gradi...

Page 52: ...lter control from the Options menu and the following dialog box is displayed Click Eject to move the Absorbance filter slide out of the instrument To insert a filter slide open the filter compartment...

Page 53: ...yed To move the plate carrier out click Eject Plate To move the plate carrier in click Insert Plate The plate carrier is automatically positioned correctly When a measurement is started the plate move...

Page 54: ...tions menu and the following dialog box is displayed Select the number of cycles e g to 200 Click Start to start the duration test If the barcode option is activated a microplate with a barcode can be...

Page 55: ...Replacing the Instrument Cover 5 1 Removal of Instrument Top Cover The instrument top cover is removed using the following procedure 1 Switch the instrument OFF 2 Remove the main power cable from the...

Page 56: ...placing the Instrument Cover 5 2 Technical Manual for sunrise RC TW TS TC BC 6F No T 137 302 Rev No 1 1 2004 10 5 Carefully remove the E Pack top cover To replace the top cover follow the instructions...

Page 57: ...lamp compartment cover by gently pulling it from the underside 4 Remove the four screws in the right hand side cover area 5 Open the plate opening lid and remove the screw in the top left hand corner...

Page 58: ...hscreen The instrument top cover with Touchscreen is removed using the following procedure 1 Switch the instrument OFF 2 Remove the main power supply cable from the socket 3 Disconnect the Touchscreen...

Page 59: ...sing through the filter the beam falls onto the fiber optic unit which distributes the available light into the 13 beams 12 measurement beams and a reference beam The fiber optic unit projects the 12...

Page 60: ...Standard Optic Instrument Transport box Fiber Optic Unit Filter Carriage Lamp Mirror Heat Absorbing Filter Optical Lens Reference Diode Channel Microplate Main Board SUNRISE TW Tuneable Wavelength Opt...

Page 61: ...ement Mask for SUNRISE ST Option The Sunrise ST instrument can perform Agglutination measurements where the optical density is measured up to 40 times across each well Agglutination Measurement Optica...

Page 62: ...or unit consists of the following serviceable parts Heat filter Air filter Mirror Optical lens To service these parts the following procedures have to be executed 6 6 1 Heat Filter and Air Filter 1 Sw...

Page 63: ...5 Removing and Replacing the Instrument Cover 4 Remove front cover 5 Remove power board 6 Disconnect ST1 ST8 ST7 ST3 ST15 ST20 and ST 21 of main board and all connections of the of power board 7 Disc...

Page 64: ...sary 6 7 Filter Guide Standard Instrument The picture below shows the main components of the filter guide Sensor Fibre Slot DC Motor Filterslide housing The filter guide is responsible to detect the f...

Page 65: ...e the main power cable from the socket 3 Remove top cover and upper EPAC see 5 Removing and Replacing the Instrument Cover 4 Remove front cover 5 Remove power board 6 Disconnect ST1 ST8 ST7 ST3 ST15 S...

Page 66: ...e top cover and upper EPAC see 5 Removing and Replacing the Instrument Cover 4 Remove front cover 5 Remove power board 6 Disconnect ST1 ST8 ST7 ST3 ST15 ST20 and ST 21 from the main board and all conn...

Page 67: ...using screws M3X12 The fiber bundle itself is not serviceable In case the fiber bundle fails it has to be changed as a complete unit For replacing this part please use to the following procedure 1 Swi...

Page 68: ...ot serviceable In case either of the lenses become damaged the transport box will need to be replaced In this case refer to chapter 7 Mechanical System The agglutination mask is also part of the trans...

Page 69: ...Replacing the Instrument Cover 4 Remove front cover 5 Remove power board 6 Disconnect ST1 ST8 ST7 ST3 ST15 ST20 and ST 21 of main board and all connections of the of power board 7 Remove the power bo...

Page 70: ...6 Optical System 6 12 Technical Manual for sunrise RC TW TS TC BC 6F No T 137 302 Rev No 1 1 2004 10 6 12 Filter Wheel Properties Dark blue Home sensor Green filter Light blue filter...

Page 71: ...al switch is not used The distance between the End and the Start optical switches is measured in steps during the machine set up The number of steps is stored in the instrument s memory The measured n...

Page 72: ...f steps After the instrument has completed the measurement the plate support is returned to the Start optical switch The instrument checks that the number of steps required to reach the Start optical...

Page 73: ...OFF 2 Remove the main power cable from the socket 3 Remove top cover and upper EPAC see 5 Removing and Replacing the Instrument Cover 4 Remove front Cover see 5 Removing and Replacing the Instrument C...

Page 74: ...No T 137 302 Rev No 1 1 2004 10 8 Remove the Transport Box carefully 9 Remove the fiber bundle from the Transport Box 10 Replace all parts in reverse order Note To avoid damage to the fiber bundle tak...

Page 75: ...ature Control Unit 1 Switch the instrument OFF 2 Remove the main power cable from the socket 3 Remove top cover and upper EPAC see 5 Removing and Replacing the Instrument Cover 4 Remove front cover se...

Page 76: ...ev No 1 1 2004 10 11 Remove the Thermo Board and disconnect all cables on the board 12 Remove the Peltier module including cooling unit 13 Remove the fans and make a note of the position of each fan s...

Page 77: ...erature control of the instrument fan 3 Power Board tuneable wavelength 6 Filter This board has the same function as the standard board but there is one more motor driver for controlling the filter ca...

Page 78: ...Diagram Standard RC The diagram below shows how the various parts of the instrument are interconnected ST 20 MAIN BOARD POWER BOARD ST 19 POWER SUPPLY Mains Input ON OFF Switch Fuses ST 15 ST 23 ST 5...

Page 79: ...N OFF Switch Fuses ST 15 ST 23 ST 5 ST 6 ST 18 ST 22 ST 11 ST 14 ST 12 ST 21 ST 7 ST 2 ST 8 ST 1 BOOT EEPROM CPU VAN RS 232 PRINTER INTERFACE TANSPORT MOTOR TRANSPORT START POSITION OPTICAL SWITCH TRA...

Page 80: ...components CPU chip Boot EPROM chip Battery A D converter Area Connector to Power Supply A D Converter Area Processor Boot EPROM Connector to printer interface Connector to Powerboard Connector to RS...

Page 81: ...ment top cover 4 Remove the Epack top cover 5 Disconnect the Power Board on connector ST20 6 Disconnect the three optical switch connectors 7 Disconnect the power supply connector on ST21 8 Disconnect...

Page 82: ...Rev No 1 1 2004 10 14 Perform the device configuration with the SunSet software as shown in the picture 15 Perform all adjustments with the SunSet software as described in 4 4 SunSet Software Service...

Page 83: ...d to control the transport motor filter carriage motor the 5 Volt voltage regulation the lamp control and for the temperature control of the instrument fan Front side Rear side Connector to CPU board...

Page 84: ...top cover 9 Remove the Epack top cover 10 Disconnect the Power Board on connector ST20 11 Disconnect the lamp connector on ST15 12 Disconnect the power on LED connector on ST23 13 Disconnect the filt...

Page 85: ...rd board but there is one more motor driver for control the filter carriage motor DC Servo Motor by TW Option and 6 Filter Option There is also a voltage regulation for the TW board Front view Rear vi...

Page 86: ...p cover 4 Remove the Epack top cover 5 Disconnect the Power Board on connector ST20 6 Disconnect the lamp connector on ST15 7 Disconnect the power on LED connector on ST23 8 Disconnect the filter tran...

Page 87: ...Disconnect the power on LED connector on ST23 8 Disconnect the filter transport motor connector on ST5 9 Disconnect the transport motor connector on ST6 10 Disconnect the Power supply connector on ST...

Page 88: ...using the following procedure 1 Switch the instrument OFF 2 Remove the main power cable from the socket 3 Remove the instrument top cover 4 Remove the Epack top cover 5 Disconnect all cable connectio...

Page 89: ...d using the following procedure 1 Switch the instrument OFF 2 Remove the main power cable from the socket 3 Remove the instrument top cover 4 Remove the Epack top cover 5 Remove the power connection s...

Page 90: ...Power Supply The power supply unit is replaced using the following procedure 1 Switch the instrument OFF 2 Remove the main power cable from the socket 3 Remove the instrument front cover 4 Remove the...

Page 91: ...etion to cpu board option cable Boot EPROM Laser Scanner The power supply unit is replaced using the following procedure 1 Switch the instrument OFF 2 Remove the main power cable from the socket 3 Rem...

Page 92: ...sunrise RC TW TS TC BC 6F No T 137 302 Rev No 1 1 2004 10 8 15 Optical Switches The following optical switches are used in the instrument Transport start position and transport end positions Optical s...

Page 93: ...8 Electronic System 2004 10 Technical Manual for sunrise RC TW TS TC BC 6F No T 137 302 Rev No 1 1 8 17 Home Position sensor of the filter wheel Circuit Diagram GND OUT A K GND...

Page 94: ...ing the following procedure 1 Switch the instrument OFF 2 Remove the main power cable from the socket 3 Remove the instrument top cover 4 Remove the Touchscreen 5 Disconnect the USB stick 6 Unscrew th...

Page 95: ...1 Replace all parts in reverse order 8 17 Converter Board The Converter Board controls the power for the backlight of the display The power supply unit is replaced using the following procedure 1 Swit...

Page 96: ...panel is replaced using the following procedure 1 Switch the instrument OFF 2 Remove the main power cable from the socket 3 Remove the instrument top cover 4 Remove the Touchscreen 5 Remove the USB s...

Page 97: ...No T 137 302 Rev No 1 1 8 21 7 WinCE Board 8 Lift the unit out carefully without damaging or shifting the gasket on the front of the screen 9 Disconnect the three connectors to the converter and the W...

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Page 99: ...ough an RS 232 C interface These interface specifications maybe either to the CCITT interface standard or to the EIA RS 232 C interface standard In case of a communication error the data transmission...

Page 100: ...able used to connect the instrument to the computer should be wired as given below INSTRUMENT COMPUTER TD connected to RD RD connected to TD GND connected to GND RTS connected to CTS DSR connected to...

Page 101: ...ce standard or to the EIA RS 232 C interface standard Implemented RS 232 C Interface Lines Shortform Signal Name Description Direction TxD Transmit Data Reader sends Data on this line Output RxD Recei...

Page 102: ...upt controlled function receives any command from the serial interface line when the command starts with STX 0x02 and it ends with ETX 0x03 CS CR 0x0D and writes it into a string otherwise the command...

Page 103: ...correctly on both the computer and the instrument Start stop synchronous system Asynchron Start bit length Stop bit length Data format Parity bit Baud Rate These parameters are checked and set using t...

Page 104: ...here are time spans of a maximum length of about 0 2 seconds during which the instrument is not able to respond to any command from the computer While the instrument is completing the measurements the...

Page 105: ...he U S A character set Use the printer manual to ensure that the DIP switches of the printer are correctly set for the U S A character set 9 11 2 Hardware Specifications The following hardware specifi...

Page 106: ...interface connector and the direction of the signals in relationship to the instrument 9 13 Table of Connections Signal pin Signal Direction 1 STROBE out 2 DATA 1 out 3 DATA 2 out 4 DATA 3 out 5 DATA...

Page 107: ...arry the 8 data bits of information The data is read in synchronization with the strobe pulse A high level indicates a logical 1 The signal must be present 0 5 microseconds before the strobe pulse is...

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Page 109: ...1 10 1 1 Introduction In order to perform some of the service and repair adjustment procedures you will need the Service Tool Kit It consists of a service plate spare screws and clips the software pac...

Page 110: ...rocedure The QC Pac 2 Service Sunrise Setup and Sunrise Diagnosis software installation is described in 4 4 SunSet Software Service tool kit is necessary 10 3 Sunrise SUNSET Software Note For Adjustme...

Page 111: ...ersion is that the Service version uses tighter tolerances then the standard version Therefore a final test using the QC Pac 2 Service version should be performed after repairing the instrument The QC...

Page 112: ...h on the instrument and the new Magellan will be downloaded automatically 4 When the screen is dark the download progress is finished 5 Switch off the instrument and remove the Flashcard 6 Insert the...

Page 113: ...rade Kit 10 6 1 General The barcode upgrade kit consists of the following Installation Manual Sunset Software Detection Suite CD Laser Scanner Screws Barcode Board Mirror Adjustment Microplate Option...

Page 114: ...trument OFF 2 Remove the main power cable from the socket 3 Remove the instrument top cover 4 Remove the front cover of the instrument 5 Install the mirror plate by attaching the two screws M 3x8 Plas...

Page 115: ...BC 6F No T 137 302 Rev No 1 1 10 7 7 Open the brown clasp of the connector on the BCR Board connect the cable and close the clasp again 8 Connect the option flex cable to the barcode board ST3 and to...

Page 116: ...se RC TW TS TC BC 6F No T 137 302 Rev No 1 1 2004 10 9 Attach the 3 labels to the rear of the instrument 10 Attach the Laser label to the reading head 11 Replace the instrument covers Laser Radiation...

Page 117: ...e RC TW TS TC BC 6F No T 137 302 Rev No 1 1 10 9 10 6 3 Software Installation and Adjustment Install the Sunset software on your computer hard disc Start the Sunset software and under Setup select Con...

Page 118: ...4 10 In the User configuration dialog box under Options select Barcode and click OK Insert all defined filter slides step by step for automatic calibration Disconnect the instrument from the Sunset so...

Page 119: ...or Sunrise RC and Options 2004 10 Technical Manual for sunrise RC TW TS TC BC 6F No T 137 302 Rev No 1 1 10 11 In the Instrument menu select Connect Instrument Connect On the Service menu select Test...

Page 120: ...l for sunrise RC TW TS TC BC 6F No T 137 302 Rev No 1 1 2004 10 Enter the single command TR0 in the command field and press the send button plate transport out TR0 Answer Place the adjustment micropla...

Page 121: ...R1 in the command field and click the send button Plate transport in TR1 Answer Enter the single command DB IBUS SEND B V in the command field and press the send button get barcode Firmware version DB...

Page 122: ...he command field and press the send button start barcode scanning Note Enter a barcode reset DB IBUS SEND B R step in the command field before entering the barcode scanning command DB IBUS SEND B S An...

Page 123: ...ess the send button drive in the Plate transport Enter the single command DB MCU POS 24 in the command field and press the send button Plate transport to BCR position Enter the single command DB IBUS...

Page 124: ...o 1 1 2004 10 10 7 Overview of Single commands TR0 Removal of the Plate transport TR1 Insert Plate transport RV Get Sunrise Version DB IBUS SEND B V get barcode version DB IBUS SEND B R reset barcode...

Page 125: ...input fuses Clean the instrument Disinfect the instrument WARNING REMOVE THE MICROPLATE BEFORE DOING ANY MAINTENANCE 11 2 Lamp Replacement Note The instrument specifications can only be guaranteed if...

Page 126: ...Replace the old lamp 7 Reconnect the lamp power connector 8 Replace the lamp into the lamp holder Please note that the lamp is held in place by three lugs between which the lamp is to be inserted Ins...

Page 127: ...lat surface 1 2 3 4 3 Using a wooden or rubber rod ensure that the ends are rounded so that the corners do not scratch the filters carefully push the filter and retaining ring out of the filter carria...

Page 128: ...re different if a standard filter 6 filter or a gradient filter is used If a method is defined using standard filters specifications for standard filters are valid If the same method is performed late...

Page 129: ...instrument 2 Place the filter carriage on a clean flat surface so that the Phillips head screws are visible 3 Remove the two screws and then remove the retaining bar that holds the retaining pins 4 Re...

Page 130: ...e power cord 2 Open the plastic cover of the fuse compartment by inserting a screw driver into the slot in the top of the cover and pushing the cover out 3 The fuse holders are located above the on of...

Page 131: ...r more of the diode lenses not being clean Therefore contact the service to clean and check the instrument Cleaning the Device Clean the outside of the device only with a dry or moist cloth If very di...

Page 132: ...d 11 6 2 Weekly Clean the cover and the plate transport with a mild detergent Caution Never use Acetone as it will damage the covers 11 6 3 Every Six Months Clean the filters using an optical cleaning...

Page 133: ...leted by the operating authority If a disinfection certificate is not supplied the instrument may not be accepted by the servicing center or it may be held by the customs authorities 11 7 1 Disinfecti...

Page 134: ...aper towel and a mild detergent or distilled water to remove all traces of the disinfectant 9 Wipe dry the outer surfaces of the instrument 10 Pack the instrument and its accessories 11 Disinfect your...

Page 135: ...and packaging waste the manufacturer is responsible for the disposal of packing material Returning Packing Material If you do not intend to keep the packing material for future use e g for transport...

Page 136: ...isposing of the instrument Caution Always disinfect the instrument before disposal Pollution degree 2 IEC EN 61010 1 Method of disposal Contaminated waste WARNING DEPENDING ON THE APPLICATIONS PARTS O...

Page 137: ...re instructions on how to easily check the performance of the instrument 12 2 Optimizing for Maximum Performance The instrument has been fully factory tested to ensure that its performance is within t...

Page 138: ...lar solution It is important to use the maximum absorbance wavelength if the absorbance curve of the sample is over a narrow wavelength band 2 After each microplate has been measured please refer to t...

Page 139: ...perfectly clean microplates 3 Do not allow dust to settle onto the solutions or the microplate if the microplate is left to stand for a time before the measurement It is recommended to use a cover fo...

Page 140: ...the influence of the solution and any variation due to the positioning of the microplate when it is being measured 12 3 1 QC PAC 2 QC PAC 2 provides an automated check of reader performance including...

Page 141: ...1 0 040 0 042 0 042 0 041 0 040 0 043 0 043 0 041 0 041 G H 0 042 0 040 0 040 0 044 0 045 0 039 0 041 0 046 0 045 0 044 0 040 0 045 H 1 2 3 4 5 6 7 8 9 10 11 12 Unacceptable Microplate 1 2 3 4 5 6 7 8...

Page 142: ...orrect as the instrument normally measures the optical density at three positions in the well and uses this average measured optical density as the optical density for the well Normal Mode 3 positions...

Page 143: ...sunrise Absorbance Reader is able to read up to 40 measurement points per well Note Instruments with option tuneable wavelength selection must not be used for agglutination measurements After the micr...

Page 144: ...the measurements for these wells Compare the average value against the highest and lowest measured values Example Measured Values 0 945 0 956 0 937 0 926 0 971 0 936 0 961 0 939 0 942 0 938 Average 0...

Page 145: ...ne a test run using the 492 nm filter and then measure the microplate at least three times For each well calculate the following the average OD value the highest and lowest values the difference betwe...

Page 146: ...and the spectrophotometer at different wavelengths and compare the results obtained Make sure that the instrument is using the accuracy measurement mode Example for a standard instrument The inaccura...

Page 147: ...from the straight line 250 microliters of each dilution are then pipetted into the microplate a minimum of at least two samples should be used for each dilution to reduce the errors caused by pipetti...

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Page 149: ...able measurement 4 4 5 Mirror and lamp adjustment 0x020A Lamp low too less light to perform a reliable measurement 4 4 5 Mirror and lamp adjustment 0x020B Timeout waiting for lamp on check power board...

Page 150: ...dient Filter detected Check filterslide coding slits 0x0601 Wrong checksum in configuration block 1 Check filter settings 4 4 8 User Configuration 0x0602 Wrong checksum in all configuration blocks Che...

Page 151: ...source not found Error message appears on the printout cover page Linearity Fail Check if filter settings corresponds with the hardware setting of the filter slide or replace filter Error Reference so...

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Page 153: ...requirements 3 3 F Filter Carriage Gradient 2 16 Standard 2 15 Filter Carriage Description 2 15 Filter Replacement 6 Filter 11 5 Gradient Filter 11 4 Standard Filter Carriages 11 3 Fuse Replacement 11...

Page 154: ...8 Q QC PAC 2 12 3 Quality Control 12 1 S Safety 1 1 Self Check Procedure 12 3 Shaking 2 18 Software for sunrise Absorbance Reader 2 19 Specifications 2 6 Standard Instrument 2 7 Standard Filter Filter...

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