Sakura Tissue-Tek Polar DM, Operating Manual

Page: 82 / 132

2-13
Sectioning
Sectioning with the Anti-Roll Rake
Use of the Anti-Roll Rake eliminates the need to flatten the section with
a brush, etc., so even a beginner can make sections relatively easily.
1. Cause the tip of the Anti-Roll Rake to lightly contact the bottom side
(approx. 3 mm from the bottom [1]) of the roughly shaved specimen
block.
Use of the Anti-Roll Rake leaves edge marks at the bottom of the tissue
sample from contact with the tip of the Anti-Roll Rake, so be sure to set
the Anti-Roll Rake in such a way that its tip contacts a blank area where
there is no tissue sample.
If the Anti-Roll Rake is too high or too low, loosen the height adjustment
screw and adjust the height according to the specimen, and then tighten
the screw again.
2. Perform sectioning, and the tip of the Anti-Roll Rake will be coupled
and move forward on the stage by pulling the section [2].
Remove the Anti-Roll Rake by holding the section with a brush, etc.
3. Once a section is made, slowly move the glass slide closer to the
section from above, and the section will be attached to the glass
slide due to temperature difference [3].
Be careful not to cut your hand with the disposable blade.
If the Anti-Roll Rake is not used, tilt it all the way to the back in the
standby position.
If the Anti-Roll Rake is not in an appropriate position, its tip may not
be cooled properly and cause the frozen block to melt while in use.
Be careful not to get injured by the sharp edges at the tip of the
Anti-Roll Rake.
Rough guide on where to contact the
Anti-Roll Rake (blank area without
tissue sample approx. 3 mm from the
bottom) Tissue
sample
Anti-Roll Rake
Stage
Glass slide Section
Anti-Roll Rake
Edges at the tip
Anti-Roll Rake adjustment screw