Nikon ECLIPSE Ni-E, Instruction Manual

Page: 113 / 186

Chapter 3
Individual Operations
99
Indiv
idua
l
Op
eratio
n
s
6
Adjusting the Diopter
The diopter adjustment ring on an eyepiece can be adjusted to match the eyesight of your right and left eyes.
A properly adjusted diopter compensates for differences in visual acuity between the right and left eyes of a person,
making binocular observation easier. It also minimizes focal deviations when switching magnification, optimizing the
performance of the objective.
Adjust the diopter settings for both eyepieces.
(1) Turn the diopter adjustment ring on the right and left
eyepieces to align the end face of the diopter adjustment
ring with the line. (This is the diopter adjustment reference
position.)
(2) Follow Steps 1 through 9 in Chapter 2 “3 Bright/Dark-field
Microscopy Procedure” to focus on the specimen using the
10x objective.
(3) Operate the nosepiece to bring the 40x objective into the
optical path, and turn the coarse focus knob and then the
fine focus knob to focus on the sample.
(4) Bring the 10x (or 4x) objective into the optical path.
(5) Look into the right eyepiece with your right eye without
operating the focus knob, and then focus on the sample by
turning the right diopter adjustment ring.
(6) Look into the left eyepiece with your left eye. Without
operating the focus knob, focus on sample by turning the left
diopter adjustment ring.
(7) Repeat Steps (3) through (6) to make sure the focus has
been adjusted properly.
Aligning the end face of the adjustment ring
with the line
IN
ND
8
ND
32
NCB
11
OUT
Focusing with 40x
50
60
70
Selecting 10x or 4x
to focus with right and left diopter adjustment ring
(1)
Line
(5)
Focus with a right diopter
adjustment ring
(6)
Focus with a left diopter
adjustment ring
Focus knob
(3)
40x
(4)
10x or 4x