Chapter 2
Microscopy Operations
77
Ph
ase Co
n
tra
st
Micro
sco
p
y
Preparation
Focus and Optical
System Adjustment
Microscopy
Phase Contrast
Microscopy Procedure
1
2
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22
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15
Bring an arbitrary Ph objective into the optical path.
Press the OBJ CW or CCW button to bring the desired
Ph objective into the optical path.
Oil immersion
When using an oil immersion type objective, apply Nikon
specified non-fluorescence immersion oil between the
specimen and the objective. (See Chapter 3 “14
Oil/Water Immersion”.)
Never apply oil between the universal condenser top
lens and the specimen. The universal condenser is
designed for dry use.
Note that an oil/water immersion type objective cannot
be used for the motorized universal condenser.
Motorized nosepiece toggling
Two objectives can be alternated by specifying an
objective pair. Objectives can be switched using the
[OBJ CW/CCW] button on the microscope. (See
Chapter 3 “11.1 Motorized Nosepiece Operation”)
Bringing desired Ph objective into optical path
P A
_
4 0 x
_ _ _ _
Z :
_ _
1 2 3 4 . 5 6 7 u m
P h 1
_ _ _
A S 3 0 . 0 m m
_ _
F S 3 0 . 0 m m
[NOSEPIECE] button, sub screen: Desired objective
Summary of Contents for ECLIPSE Ni-E
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