Chapter 3
Individual Operations
147
Indiv
idua
l
Op
eratio
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s
(5) Adjust the camera’s white balance.
To adjust the white balance, press the WB button while
capturing an image of a clear section of the specimen
slide. (For fluorescent photomicrography, Nikon
recommends adjusting the white balance under normal
bright-field microscopy conditions before capturing
images.)
NCB filter
The Ni-E's filter cassette is equipped with an NCB filter for
color temperature conversion.
(6) Align the specimen.
(7) Readjust the focus onto the target.
(8) Adjust the image brightness using the camera exposure compensation function.
(9) Check the image using the DS-L3 [PAUSE] button.
(10) If the image is acceptable, press the CAPTURE button to save the image.
(For details, see the instruction manual provided with the camera.)
Number of images captured
Serigraphy is not available because the CAPTURE button
is designed to capture only one image.
ZOOM
Attaching a camera to the zooming port allows to zoom in an image.
■
Motorized DSC Zooming Port
The final magnification of the motorized DSC zooming port
can be from 0.6x up to 2.0x.
Use the CW/CCW button of the ergo controller for a zoom
operation. A zoom magnification is displayed in 0.1x
increments.
Operation button of the ergo controller
The CW/CCW button function of the ergo controller has
not been set “to zoom” as default. Reallocate the function
of the ergo controller operation buttons using DS-L3. (See
“23 Operation on DS-L3” - “23.1 Setting Up the
Microscope - (3) Configuring the Button Functions” for
details.) (See “20 Using the Ergo Controller”.)
Operation on DS-L3 (See “23.2 Controlling the Microscope”.)
Z Speed
FL cube OBJ
Fl shutter
B/F/R
EPI all
XY Speed
Zoom operation
P A
_ _
2 x
_ _ _ =
X :
_ _ _ = =
0 . 0 0 0 u m
Z M : 1 . 0 x
_ _ _
Y :
_ _ _ _ _
0 . 0 0 0 u m
Summary of Contents for ECLIPSE Ni-E
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