Chapter 2
Microscopy Operations
54
Differential
Interference Con
trast
Microscopy
Preparation
Focus and Optical
System Adjustment
Microscopy
Differential Interference
Contrast Microscopy
1
2
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23
■ ■
7
Remove the polarizer, analyzer, lambda plate, and DIC slider from the optical path.
Loosen the polarizer rotating clamp screw, turn the upper
portion of the rotating polarizer unit in the clockwise
direction to remove the polarizer from the optical path.
If the analyzer slider or lambda plate slider is in the
optical path, pull it out to the first click-stop position and
remove it from optical path.
Pull out the DIC slider at the objective 10x position from
the optical path. (It is easily removed after procedure [8]
is performed.).
7
5
3
7
0
1
JAPAN
D - D P
Polarizer removed from optical path
1
2
A
λ
10x
Removing the DIC optical element
from the optical path
Focus and Optical System Adjustment
8
Bring the 10x objective into the optical path.
Press the OBJ CW or CCW button to bring the 10x
objective into the optical path.
Pressing the OBJ CW button turns the nosepiece by one
address in the clockwise direction (as viewed from
above), while the OBJ CCW button turns the nosepiece
in the counterclockwise direction.
Restrict movement of motorized nosepiece
A function to prevent the objective from touching the
specimen when the motorized nosepiece is rotated is
available. (See Chapter 3 “11.1 Motorized Nosepiece
Operation”.)
•
Stop nosepiece rotation by Z position
Disables the nosepiece rotation if the elevating section
is above the specified position.
The configured upper limit is applied to all the objective
addresses.
•
Nosepiece
reverse
rotation
Prohibits rotating directly from sextuple/septuple
nosepiece address 1 to adjacent address 6/7.
Intelligent nosepiece
Manually switch the objective since motorized
operation is not available for the intelligent nosepiece.
On DS-L3, only information display is provided.
Bringing 10x in optical path
with [OBJ CW/CCW] button
P A
_
1 0 x
_ _ _ _
Z :
_ _ _ _ _
0 . 0 0 0 u m
O P E N
_ _
A S 3 0 . 0 m m
_ _
F S 3 0 . 0 m m
[NOSEPIECE] button, sub screen: [10x]
Polarizer rotation
clamp screw
DIC slider of 10x
objective
(or dummy slider)
Analyzer
slider
Lambda
plate
Summary of Contents for ECLIPSE Ni-E
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Page 24: ...Chapter 1 Components 10 Components ...
Page 29: ...Chapter 2 Microscopy Operations 15 Before Microscopy Operation Flowchart ...
Page 30: ...Chapter 2 Microscopy Operations 16 Before Microscopy Operation Flowchart ...
Page 31: ...Chapter 2 Microscopy Operations 17 Before Microscopy Operation Flowchart ...
