Chapter 3
Individual Operations
105
Indiv
idua
l
Op
eratio
n
s
Aperture diaphragm at phase contrast microscopy
When using the NI-CUD-E for phase contrast microscopy, fully open the aperture diaphragm. Stopping down the
aperture diaphragm may block the PH module's annular diaphragm, disabling phase contrast microscopy.
■
Condenser focus knob
On the motorized universal condenser, the condenser focusing unit is integrated with the condenser and a condenser
focus knob is attached on the left and right sides of the focusing unit.
They are used to adjust the condenser position so that the light passing through the condenser forms an image at the
correct position (center of the optical path) on the surface of the sample. (See “7 Focusing and Centering the
Condenser”.)
■
Condenser
centering
screw
IN
OUT
There are two condenser centering screws at the front of the
condenser.
They are used to adjust the condenser position so that the
light passing through the condenser forms an image at the
correct position (center of the optical path) on the surface of
the sample. Adjust by inserting the provided hex driver (2
mm across flats) into the centering screw hole and turning
the internal centering screw. (See “7 Focusing and Centering
the Condenser”.)
■
PH module centering screws
There are PH module centering screws (x2) at the rear of the
condenser. The screws are equipped with a knob. To adjust
the centering screw, pull the knob out and set it horizontally
and then push it into the screw. Keep the knob pulled down
when not used. (See Chapter 2, “6 Phase Contrast
Microscopy Procedure
-
14
Center the PH module”.)
Motorized universal condenser
viewed from left rear side
Operation from DS-L3 (See “23.2 Controlling the Microscope”.)
9.3
Using the NI-CUD Universal Condenser (Dry)
The NI-CUD is a dry type manual universal condenser that supports various microscopy methods.
■
Optical
module
The turret of the universal condenser has one empty hole and six holes for attaching optical modules. The hole at
address 1 is empty and no optical module can be attached here. This is the position to bring into the optical path during
bright-field microscopy. Attach the following optical modules at addresses 2 to 7. (See Chapter 1, “3 Assembly
Method
-
18 Attach the condenser
-
■
Attaching the optical module to the universal condenser” in the
“Assembly/Maintenance” instruction manual.)
DIC module (required for differential interference contrast microscopy)
DIC module includes [D-C DIC N1 DRY], [D-C DIC N2 DRY], and [D-C DIC NR DRY].
Select the DIC module appropriate for the microscopy method and objective. If the combination is not correct,
differential interference contrast image cannot be obtained or the contrast decreases significantly. Depending on the
purpose of observation, dedicated modules for higher contrast or resolution are available. Note, however, that in
principle the contrast contradicts the resolution of the differential interference contrast image (the higher the
contrast, the lower the resolution). (See the table in Chapter 1, “3 Assembly Method - 18 Attach the
condenser - Combinations of the DIC Slider on the Objective and the DIC Module on the Condenser (when
NI-CUD-E/NI-CUD universal condenser is used)” in the “Assembly / Maintenance” instruction manual.)
Condenser centering screw hole
PH module
centering knob
(on both sides)
Summary of Contents for ECLIPSE Ni-E
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Page 24: ...Chapter 1 Components 10 Components ...
Page 29: ...Chapter 2 Microscopy Operations 15 Before Microscopy Operation Flowchart ...
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