Chapter 2
Microscopy Operations
42
Ep
i-fluo
resce
n
ce
Micro
sco
p
y
Preparation Focusing
Microscopy
Epi-fluorescence
Microscopy Procedure
1
2
3
4
5
6
7
8
9 10 11 12 13 14
■
■
■
■
■
Switch from Epi-Fluorescence Microscopy to Bright-field Microscopy
(1)
When switching from epi-fluorescence to bright-field
microscopy, close the epi-fluorescence illumination path
shutter and block the illumination of the mercury lamp.
The shutter is closed when the [FL SHUTTER] button is
lit.
ND
8
ND
32
OUT
IN
NCB
11
Closing shutter
(2)
Rotate the epi-fluorescence cube turret to bring turret
address 1 into the optical path. (If a filter cube is in
address 1, remove it in advance.)
ND
8
ND
32
OUT
IN
NCB
11
Removing the filter cube
P F
_ _
2 x
_ _ _ _
Z :
_ _ _ _ _
0 . 0 0 0 u m
O P E N
'
C
_ _ _ _ _
N D
_
1
'
C
_ _
E P I : C
Empty position Shutter closed
(3)
Press the dia-illumination ON/OFF switch to turn on the
lamp.
Dia-illumination lamp ON
(4)
Focus and center the condenser. (See Chapter 3 “7
Focusing and Centering the Condenser”.)
For details on other operations and precautions, see “3
Bright/Dark-field Microscopy Procedure”.
Lit
Summary of Contents for ECLIPSE Ni-E
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