Chapter 3
Individual Operations
119
Indiv
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Op
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Using double layered epi-fluorescence cube turrets
Two epi-fluorescence cube turrets can be used by layering them on top of each other.
Attach an attachment using a laser as an illuminator on the 1st layer, which should be used for light stimulation when
using a double-decked turret. On the 2nd layer, an epi-fluorescence attachment is attached to be used for the
epi-fluorescence microscopy. This double-decked configuration is supplied as a separate system, so this document
does not mention the description on that system.
Basically this document describes the epi-fluorescence cube turret on the 1st layer but mentions the one on the 2nd
layer as needed. The front display panel indicates “-2” on the back of the filter cube indication to indicate the
epi-fluorescence cube turret on the 2nd layer.
Switch the epi-fluorescence cube turret on the 2nd layer from a FUNCTION button allocated. (See “23 Operation on
DS-L3” - “23.1 Setting Up the Microscope - (3-1) Ni-E microscope button” for details.)
Configuring the speed of the epi-fluorescence cube turret
Both epi-fluorescence cube turrets on the 1st and 2nd layers are configured for “high speed” operation upon shipment,
but can also be configured for “low speed” operation for use with thick dichroic mirrors. (See “23 Operation on DS-L3” -
“23.1 Setting Up the Microscope - (7-1) FL Turret Drive Speed” for details.)
Interlocked operation of the epi-fluorescence cube turret
The switching of the motorized epi-fluorescence cube turret can be interlocked when pressing the [CAPTURE] button
to capture an image. See “19.2 Interlocking Function” for conditions for interlocking and interlocked operations.
Intelligent epi-fluorescence cube turret/epi-fluorescence cube turret operation
Manually rotate the cube switchover turret to switch the filter
cube between intelligent epi-fluorescence cube turret and
manual epi-fluorescence cube turret. The filter cube on the
optical path is indicated by the address displayed on the front of
the turret.
Make sure that the turret clicks to a stop so that the filter cube
can be properly brought into the optical path when switching the
turret.
The front slot cover for a filter cube replacement has a window
for inserting a filter cube nameplate. You can check the
information on the filter cube placed in the optical path when the
nameplate is kept inserted into the window of the address to
which the filter cube is attached.
You can check the filter cube on the optical path on the display
panel of the front of the Ni-E microscope if the attachment
information on the filter cube has been set, as done with the
motorized epi-fluorescence cube turret, when using an
intelligent epi-fluorescence cube turret.
For information on attaching filter cubes to the turret, see
Chapter 1 “3 Assembly - 10 Attach the epi-fluorescence cube
turret and epi-fluorescence attachment -
■
Attaching the filter
cube” in the “Assembly/Maintenance” instruction manual.
1
2
A
λ
2
1
2
3
4
5
6
Turning the turret
Address indication
on turret
Cube switchover turret
Filter cube nameplate window
Summary of Contents for ECLIPSE Ni-E
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