Chapter 2
Microscopy Operations
43
Ep
i-fluo
resce
n
ce
Micro
sco
p
y
Preparation Focusing
Microscopy
Epi-fluorescence
Microscopy Procedure
1
2
3
4
5
6
7
8
9 10 11 12 13 14
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Switch from Differential Interference Contrast Microscopy to Epi-Fluorescence Microscopy
Epi-fluorescence and differential interference contrast methods can be used concurrently to cover the
shortcomings of each. For example, when locating the target, differential interference contrast microscopy can be
used instead of epi-fluorescence microscopy which tends to cause decoloration of the specimen.
This section provides tips for switching from differential interference contrast microscopy to epi-fluorescence
microscopy when devices required for epi-fluorescence microcopy and differential interference contrast
microscopy are attached. Precautions on concurrent use of the two methods are also noted. Also see “5
Differential Interference Contrast Microscopy Procedure”.
(1)
When turning on the power switch at the beginning of “5
Differential Interference Contrast Microscopy”, keep the
power of the HG precentered fiber illuminator for
epi-fluorescence illumination off.
Check that the epi-fluorescence illumination path
shutter is closed.
The shutter is closed when the [FL SHUTTER] button
is lit.
ND
8
ND
32
OUT
IN
NCB
11
Closing shutter
(2)
All optical elements required for differential interference
contrast microscopy are removed from the optical path
in procedures 6 and 7 in “5 Differential Interference
Contrast Microscopy Procedure”.
At the same time,
•
Remove the filter cube from the optical path and
set in empty position.
ND
8
ND
32
OUT
IN
NCB
11
Removing the filter cube
•
Also remove the FL/DIC analyzer slider used when
concurrently performing
epi-fluorescence/differential interference contrast
microscopy from optical path.
The slider is removed from the optical path by
pulling it out by one click-stop from the depressed
state.
Focus on the sample by bright-field and adjust the
optical system in this state.
1
2
A
λ
F. STO
P
2
3
4
5
6
50
60
70
Removing the FL/DIC analyzer slider
(3)
To adjust the orientation of the polarizer and analyzer in
procedure 14 in “5 Differential Interference Contrast
Microscopy Procedure”, use the FL/DIC analyzer slider
attached to the epi-fluorescence cube turret rather than
the analyzer slider attached to the nosepiece. The
analyzer enters the optical path by pushing the slider to
the second click-stop position.
NI-FA FL/DIC
Analyzer Slider
Lit
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