Chapter 2
Microscopy Operations
24
Brig
h
t/Dark-field
Micro
sco
p
y
Preparation
Focus and Optical
System Adjustment
Micro-
scopy
Bright/Dark-field
Microscopy
1
2
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19
9
Focus on the sample.
(1)
Looking through the eyepiece, adjust the brightness for
optimum brightness by rotating the dia-illumination
brightness control knob.
(2)
Looking through the eyepiece, focus on the sample by
rotating in the order of the coarse focus knob and then
the fine focus knob. (See Chapter 3 “4.1 Proper
Focusing Procedure”.)
Z-RESET
The Z-axis coordinate value can be reset to zero.
This is useful when you wish to use the current
position as a reference in adjusting the focus. (See
Chapter 3 “4.3 Resetting the Z-axis Coordinate”.)
Refocusing
This operation moves the Z-axis position to the
retracting position or returns it to the original position.
(See Chapter 3 “4.4 Refocusing”.)
Brightness adjustment
Brightness adjustment
Focusing using the focus knob
P A
_
1 0 x
_ _ _ _
Z :
_ _ _ _ _
1 . 2 3 4 u m
O P E N
_ _
A S 3 0 . 0 m m
_ _
F S 3 0 . 0 m m
Z-axis
coordinate
Note on controlling the focus knobs
Avoid the following action, which can cause equipment
malfunction.
•
Rotating the right and left focus knobs in opposite
directions.
Do not turn the focus knobs in opposite direction!
Summary of Contents for ECLIPSE Ni-E
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