Chapter 2
Microscopy Operations
75
Ph
ase Co
n
tra
st
Micro
sco
p
y
Preparation
Focus and Optical
System Adjustment
Microscopy
Phase Contrast
Microscopy Procedure
1
2
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22
■
■
13
Bring the PH module (Ph1) into the optical path.
Press the FUNCTION button 1/2 to rotate the condenser
turret to display [Ph1] on the display panel at the front
side of the Ni-E main body. The PH module for the turret
is brought into the optical path.
Button 1 is for counterclockwise rotation and button 2 is
for clockwise rotation.
(Chapter 3 “18 Phase Contrast Microscopy” - “18.2 Using
Optical Elements”.)
IN
ND
8
ND
32
NCB
11
OUT
Bringing the PH module (Ph1) into optical path
P F
_
1 0 x
_ _ _ _
Z :
_ _
1 2 3 4 . 5 6 7 u m
P h 1
_ _ _
A S 3 0 . 0 m m
_ _
F S 3 0 . 0 m m
GIF filter
The GIF filter (green interference filter) improves the
contrast when placed in the optical path. It is inserted
above the field lens.
[CONDEN.] button, sub screen: [Ph1]
Optical module
in optical path
[Ph1]
Summary of Contents for ECLIPSE Ni-E
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