Chapter 2
Microscopy Operations
61
Differential
Interference Con
trast
Microscop
y
Preparation
Focus and Optical
System Adjustment
Microscopy
Differential Interference
Contrast Microscopy
1
2
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19 20 21 22 23
■ ■
18
Adjust the aperture diaphragm
Press the DIA aperture diaphragm button to adjust the
aperture. The aperture should be adjusted to about 70 to
80% of the numerical aperture of the objective. (See
Chapter 3 “8 Adjusting the Aperture Diaphragm”.)
Appropriate size of the aperture
diaphragm
Normally, the appropriate size of the aperture
diaphragm is 70 to 80% of the numerical aperture of
the objective. Since an excessively small aperture
diaphragm opening will degrade the image
resolution, Nikon does not recommend setting the
aperture diaphragm to less than 60% of the
numerical aperture of the objective.
Adjustment timing for the aperture
diaphragm
Be sure to adjust the aperture diaphragm each time
you change the objective.
ND
8
ND
32
OUT
IN
NCB
11
Adjusting aperture diaphragm
P A
_
4 0 x
_ _ _ _
Z :
_ _
1 2 3 4 . 5 6 7 u m
N 1
_ _ _
A S 1 2 . 8 m m
_ _ _
F S 3 0 . 0 m m
Appropriate size of the aperture diaphragm
[A. STOP] button, sub screen: DIA aperture
diaphragm adjustment
19
Focus on the sample
Focus on the sample by rotating the focus knob. (See
Chapter 3 “4.1 Proper Focusing Procedure”.)
Focusing using the focus knob
P A
_
4 0 x
_ _ _ _
Z :
_ _
1 2 3 5 . 5 5 5 u m
N 1
_ _ _
A S 1 2 . 8 m m
_ _ _
F S 3 0 . 0 m m
Z-axis
coordinate
Aperture
diaphragm image
Pupil of objective
70
～
80
100
Summary of Contents for ECLIPSE Ni-E
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