Nikon ECLIPSE Ni-E, Instruction Manual

Page: 88 / 186

Chapter 2
Microscopy Operations
74
Ph
ase Co
n
tra
st
Micro
sco
p
y
Preparation
Focus and Optical
System Adjustment
Microscopy
Phase Contrast
Microscopy Procedure
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 ■ ■
10
Perform diopter adjustment.
Look into the right eyepiece with your right eye and the
left eyepiece with your left eye. Turn the diopter
adjustment ring of each eyepiece to focus on the
specimen. Do not use the focus knobs. (See Chapter 3
“6 Adjusting the Diopter”.)
50
60
70
Adjusting the diopter
11
Adjust the interpupillary distance.
Look into both eyepieces and rotate the binocular part to
adjust the binocular part's opening until the fields of view
for the right and left eyes coincide.
Tip on adjusting the interpupillary distance
For easy adjustment, look into the eyepiece as if you
were looking at a distant object.
50
60
70
Adjusting the interpupillary distance
12
Focus and center the condenser.
(1) Press the DIA field diaphragm button to close the field
diaphragm to a minimum and look into the eyepiece.
Focus on the field diaphragm image using the
condenser focus knob, and then adjust the condenser
centering screws with a tool to center the diaphragm
image within the field of view.
(2) Bring the 40x objective into the optical path to check the
focus and centering of the field diaphragm image. Make
adjustments as necessary.
(3) Press the DIA field diaphragm button and adjust the
field diaphragm image so that its size is close to the
field of view.
(See Chapter 3 “7 Focusing and Centering the
Condenser”.)
Focusing and centering the condenser
Field diaphragm
image
Eyepiece
field ring
Field diaphragm
image
Eyepiece
field ring
Coincide the field
of view for the
right and left