Chapter 2
Microscopy Operations
20
Brig
h
t/Dark-field
Micro
sco
p
y
Preparation
Focus and Optical
System Adjustment
Micro-
scopy
Bright/Dark-field
Microscopy
1
2
3
4
5
6
7
8
9 10 11 12 13 14 15 16 17 18 19
3
Adjust the optical path in the tube to direct 100% of the light to the binocular section.
Press the BINO optical path button on the front of the
main body.
There are three buttons on the front of the main body for
switching the optical path in the tube. The pressed button
is lit and that optical path status is set.
•
BINO
optical path
button
:
100% to binocular
•
FRONT optical path
button
: 100% to tube adapter
•
REAR optical path
button
:
100% to rear port
Binocular 100% with [BINO] button
P A
_ _
2 x
_ _ _ _
Z :
_ _ _ _ _
0 . 0 0 0 u m
B I N O
_ _ _ _
Z M : 1 . 0 x
_
F S 3 0 . 0 m m
[BINO] button: Binocular optical path 100%
4
Lower the condenser slightly from the uppermost position.
Turn the condenser focus knob until the condenser is
positioned at the upper limit (where it clicks to a stop),
and then lower it a little.
When used in combination with the
motorized XY stage
Be sure to perform this operation when using in
combination with the motorized XY stage. When the
condenser is left at the upper limit, the sample holder
may touch the condenser when moved.
IN
ND
8
ND
32
NCB
11
OUT
Lowering the condenser slightly
from uppermost position
using the condenser focus knob
Summary of Contents for ECLIPSE Ni-E
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