Chapter 3 Individual Operations
3.18 Image Capture
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3.18
Image Capture
Images can be captured by attaching a camera head to the ergonomic binocular tube or
trinocular eyepiece tube.
For more detailed discussion of this topic, refer to the operating manual provided with the
camera head or camera control software.
Proper adjustment of light intensity and focus on the microscope side are important for obtaining
clear images. Listed below are key considerations in capturing clear images.
3.18.1
Adjusting light intensity
Lamp voltage: When the 50i is used in applications for which accurate color reproduction is
critical, set the brightness control knob at a midpoint and use ND filters to make
brightness adjustments.
Filter:
Place a commercially available color compensation filter on the filter holder at the
microscope base, as necessary.
3.18.2
Adjusting the condenser
•
Always focus and center the condenser.
•
Center the annular diaphragm for phase contrast microscopy.
•
For normal operations, set the diaphragm aperture to 70 to 80% of the N.A. of the
objective.
3.18.3
Confirming the photomicrographic range
The image on the monitor represents the photomicrographic range.
3.18.4
Confirming focus
Check focus by viewing through the eyepiece and viewing the monitor. If the focal positions for
the two images differ, adjust the focal position adjustment screw at the camera port.
