Nikon 50i, Instructions Manual

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Contents
12
3.4.4 Adjusting the rotating torque of the coarse focus knob ................................... 41
3.4.5 How to refocus .......................................................................................... 41
3.5 XY Stage Motion ............................................................................................... 42
3.5.1 Prohibited action........................................................................................ 42
3.5.2 Knob rotation direction and stage motion direction ........................................ 42
3.5.3 Adjusting the knob heights ......................................................................... 42
3.5.4 Adjusting the knob rotation torque............................................................... 42
3.6 Diopter Adjustment ........................................................................................... 43
3.7 Interpupillary Adjustment .................................................................................. 44
3.8 Adjusting the Observation Position ...................................................................... 44
3.9 Adjusting the Condenser Position ........................................................................ 45
3.10 Adjusting the Aperture Diaphragm ...................................................................... 46
3.10.1 Adjusting the aperture diaphragm opening using the condenser scale .............. 46
3.10.2 Adjusting the aperture diaphragm opening using the centering telescope
(optional) ................................................................................................. 46
3.11 Selecting a Condenser ....................................................................................... 47
3.12 Adjusting the Field Diaphragm ............................................................................ 47
3.13 Oil Immersion Operation .................................................................................... 48
3.14 Water Immersion .............................................................................................. 49
3.15 Using the Cytodiagnostic Unit ............................................................................. 50
3.15.1 Magnification switching............................................................................... 50
3.15.2 Marking specimens .................................................................................... 50
3.16 Fluorescence Observation................................................................................... 51
3.16.1 Warning.................................................................................................... 51
3.16.2 Shutter of the Epi-fl attachment .................................................................. 51
3.16.3 Light shielding plate of the Epi-fl attachment................................................. 51
3.16.4 Field diaphragm of the Epi-fl attachment ...................................................... 51
3.16.5 Switching excitation methods ...................................................................... 52
3.16.6 Inserting and removing the filter cubes ........................................................ 52
3.16.7 ND filters of the Epi-fl attachment................................................................ 53
3.17 Selecting Fluorescent Filters ............................................................................... 54
3.17.1 Selecting excitation filters (EX filters)........................................................... 55
3.17.2 Selection of barrier filter (BA filter) .............................................................. 55
3.17.3 Replacing excitation and barrier filters.......................................................... 57
3.17.4 Filter cube internal spacers ......................................................................... 57
3.18 Image Capture ................................................................................................. 58
3.18.1 Adjusting light intensity.............................................................................. 58
3.18.2 Adjusting the condenser ............................................................................. 58
3.18.3 Confirming the photomicrographic range ...................................................... 58
3.18.4 Confirming focus ....................................................................................... 58
3.18.5 Making adjustments to keep out extraneous light .......................................... 59
3.18.6 Anti-vibration measures.............................................................................. 59
3.18.7 Fluorescence photomicrography................................................................... 59
Chapter 4 Assembly ...........................................................................................60
Chapter 5 Replacing Consumables ........................................................................71
5.1 Replacing the lamp (for the 50i).......................................................................... 71
5.2 Recharging the battery (for the 55i) .................................................................... 72