Chapter 3 Individual Operations
3.17 Selecting Fluorescent Filters
57
3
3.17.3
Replacing excitation and barrier filters
Excitation and barrier filters can be removed from
the filter cube and replaced. (Dichroic mirrors
cannot be dismounted from the filter cube.)
Excitation filters are screw-in filters.
Barrier filters are slide-in filters. Align the
projection on the barrier filter with the groove on
the filter cube and turn clockwise approximately 30
degrees to secure in place.
3.17.4
Filter cube internal spacers
The filter cubes listed below cannot be inserted
directly into the Epi-fl attachment. Instead, as
described below, an internal spacer must first be
removed or reversed.
(1)
Place the filter cube on the work surface with
the excitation filter facing up.
(2)
Unscrew the ring retaining the excitation
filter.
(Be careful to avoid dropping the filter.)
(3)
Remove the spacer inside the ring.
(4)
Remove or reverse the spacer as appropriate
for the particular filter cube type. The actions
suitable for various filter cubes are given
below.
(5)
Reattach the ring.
- Filter cubes requiring spacer removal
•
UV-2A
•
UV-2B
- Filter cubes requiring spacer reversal
•
DAPI
•
FITC
•
GFP-L
•
GFP-B
•
TRITC
•
Tx-Red
Barrier filter
Excitation
filter
Turn about 30
°
to secure in place.
Spacer
Filter cube
Ring
