Chapter
2
Microscopy
2.3 Microscopy with Epi-fl Attachment Mounted
31
2
10
Switch to the desired objective
and view the specimen.
•
Refocus.
•
Use the ND filters of the Epi-fl attachment to
adjust brightness.
•
Adjust the field diaphragm so that it extends
slightly beyond the field of view.
•
When using an oil immersion type objective,
apply immersion oil between the specimen
and the objective.
⇒
P.51
⇒
P.48
11
To return to bright-field
microscopy.
•
Close the shutter of the Epi-fl attachment and
block the light emitted by the mercury lamp.
•
Turn on the microscope power switch to turn
on the diascopic light source.
•
Turn the filter cube switching knob and move
the position without a filter cube into the
optical path.
12
Turn off all power switches after
completing observations.
Use the ND filters to adjust brightness.
Select the desired objective.
Close the
shutter.
Press the switch to
the “|” position.
Press the power switch to
the “
◯
” position.
