Chapter 3 Individual Operations
3.6
Diopter
Adjustment
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3
3.6
Diopter Adjustment
Diopter adjustment compensates for differences in visual acuity between the right and left eyes,
improving binocular observation. It also minimizes focal deviations when switching objectives.
Adjust diopter settings for both eyepieces.
(1)
Turn the diopter adjustment ring of each
eyepiece and align the end face of the diopter
adjustment ring with the line. (This is the
diopter adjustment reference position.)
(2)
Perform steps 1) to 10) in “2.1 Bright-Field
Microscopy” to focus on the specimen with
the 10x objective.
(3)
Set the 40x objective in the optical path.
Using the coarse/fine focus knobs, focus on
the specimen.
(4)
Set the 4x or 10x objective in the optical
path.
(5)
Focus on the specimen using the diopter
adjustment rings instead of the coarse/fine
focus knobs. When making focus
adjustments, be sure to look through the
right eyepiece with your right eye and the left
eyepiece with your left eye.
(6)
Perform steps (3) through (5) twice.
Use this for focal
adjustment.
Set the
magnification to
10x and observe
with the right eye.
Line
Reference position for diopter adjustment
Use this for focal adjustment.
Set the 40x
objective in
the optical
path.
Observe with
the left eye.
Use this for focal adjustment.
