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1.4
Measurement Functions of the Instrument
1.4.1
Working Modes of Measurement
1. Wavelength scan mode. At a fixed excitation wavelength a spectrum is obtained in which the
fluorescent intensity of a sample varies with wavelength.
2. Time scan mode. At a fixed excitation wavelength, a spectrum is obtained in which the
fluorescent intensity of a sample varies with time.
3.Quantitative analysis mode. According to the quantitative relationship of fluorophotometry
F=KC, under a given experimental condition, fluorescent intensity (F) is directly proportional to the
concentration (C) of a sample component to be determined. By measuring the fluorescent intensity
of a standard solution, K is acquired by curve fitting. Then measure the fluorescent intensity of an
unknown sample. From a quasi-linear equation calculate the concentration of the component to be
determined in the unknown sample. A calibration can also be made by standard curve method or
standard addition method.
A concentration measurement can be made through direct reading from the instrument by
single-point standard calibrating method.
1.4.2
Modes of Data Measurement
1. Measuring with a single instrument. By using the functions of the keys and the functional
indicator lights on the instrument panel, read the measured data on the display window.
2. Measuring with an on-line computer. The instrument is connected with a computer through a
communication connector. The instrument control, the parameters setting, and real-time
measurement are all carried out by a computer software system. The software possesses powerful
functions of spectrum processing, data processing and storage.
Note: For different modes of data measurement, the number of usable working modes and the
convenience of use differ somewhat.
1.4.3
Self-checking and Self-correcting
1. Self-checking and self- correcting function of the instrument
The instrument will automatically initialize after startup, including self-checking and
wavelength correcting. Self-checking contains on-line condition check, excitation source check,
database module check, DAC module check, PMT module check and motor module check. The
result of each check will be printed on the LCD or in the software window when connected to
computer. Users can check out problems refer to table 1-2. Wavelength correcting program will
guarantee the accuracy of wavelength.
1
.
Appearance and Performances of the Instrument
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
