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2.3
Measurement
After preheating for 30 minutes, the instrument can be used for measurement operation. The
operating methods of the two measuring modes (measuring with a single instrument, measuring
with an on-line computer) of the instrument are separately different.
When the Fluorospectrophotometer main unit i s connected with a computer, the
selection of the different measuring modes and the operating methods are seen in detail in Section 7,
Section 8 and Section 9 of Part Two.
This section is about the operation of quantitative analysis using a single instrument for
measurement.
2.3.1
Pouring the Sample Solution into Sample Cell
Pour the sample solution to be measured into the sample cell up to about two thirds of height.
Use filter paper to remove the solution left on the outside surface of the sample cell. Place it
carefully into the sample cell holder.
Note: The sample cell is made of quartz material. When using, should take care to protect the
window of light transmission. When taking the sample cell out or placing it into the holder with
hand, should hold the sample cell diagonally with fingers.
2.3.2
Quantitative Analysis in Measurement Mode with a Single Instrument
Measuring mode with a single instrument is mainly used in the working mode for quantitative
analysis. There are two methods, that is multipoint method of standard sample and single-point
method of standard sample. The two operating methods are as follows.
1.
Measuring procedures by Multipoint Method of Standard Sample
Prepare a series of standard solutions (including blank solution). After finishing the preparation
of the solution of unknown sample, operate as the following sequence.
(1) Wavelength Adjustment. Press the “Mode” key to enter Wavelength Mode. “Current
Wavelength: ***nm” shows current wavelength of emission monochromator. Use “Adj 0” key or
the “Normalize” key to adjust target value. Press “Print” key after adjustment(See 2.1.2 for detail).
The wavelength will be automatically set to target value.
(2) PMT Level Adjustment. Press the “Mode” key into Fluorescence Value Mode. Put the
standard solution of maximal concentration into sample cell. Then press “Mode” to enter PMT level
adjust. “Current PMT level: **” shows the current PMT level. The higher the level is, the more
sensitive it will be. Use “Adj 0” key or the “Normalize” key to adjust the PMT level. Press “Print”
key after adjustment. The PMT level will be automatically set to target value. Adjust the PMT level
to make sure the fluorescence value of the maximal concentration standard solution is set between
200-400.
(3) Zero Adjustment. Place the blank solution in the light path. Press the “Mode” key into
Fluorescence Value Mode. Wait till the value is stable. Press “Adj 0” to adjust the fluorescence
2
.
Operation of the Instrument
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
