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value to zero(±0.1).
Attention: Zero adjustment is not allowed in the following measurement.
(4) Measurement for Standard Sample. Place a series of standard solutions (from dilute to
concentrated) in the light path one by one. After the fluorescence value is stable, record the value.
Fit a working curve equation according to “Data Processing with Microsoft Excel 2000 Software”
provided by Appendix.
(5) Measurement for Unknown Sample. Place the solution of the unknown sample in the
light path. After the fluorescence value is stable, record the value. Acquire the concentration of the
unknown sample from the working curve equation.
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Measuring Procedures by a Single-point Method of Standard Sample
Prepare one standard solution and one blank solution each. After finishing the preparation of
unknown sample solution, operate as the following sequence.
Note: The concentration of standard sample should close to that of sample to be measured as
far as possible.
(1) Wavelength Adjustment. Press the “Mode” key to enter Wavelength Mode. “Current
Wavelength: ***nm” shows current wavelength of emission monochromator. Use “Adj 0” key or
the “Normalize” key to adjust target value. Press “Print” key after adjustment(See 2.1.2 for detail).
The wavelength will be automatically set to target value.
(2) PMT Level Adjustment. Press the “Mode” key into Fluorescence Value Mode. Put the
standard solution of maximal concentration into sample cell. Then press “Mode” to enter PMT level
adjust. “Current PMT Level: **” shows the current level. The higher the level is, the more sensitive
it will be. Use “Adj 0” key or the “Normalize” key to adjust the PMT level. Press “Print” key after
adjustment. The PMT level will be automatically set to target value. Adjust the PMT level to make
sure the fluorescence value of the maximal concentration standard solution is set between 200-400.
(3) Zero Adjustment. Place the blank solution in the light path. Press the “Mode” key into
Fluorescence Value Mode. Wait till the value is stable. Press “Adj 0” to adjust the fluorescence
value to zero(±0.1).
Attention: Zero adjustment is not allowed in the following measurement.
(4) Direct-reading of Concentration. Press the “Mode” key to enter Concentration Input Mode.
When the LCD shows “Input Concentration: ”, use “Adj 0” key or the “Normalize” key to adjust the
value to be the concentration of standard solution. Then press the “Print” key to calculate the
concentration factor and switch to Concentration Mode.
(5) Measurement for Unknown Sample. Place the unknown sample in the sample cell. The
value on the display window is the concentration of the unknown sample in Concentration Mode.
Press the “Print” key to print the current data if connected to a dedicated serial interface printer.
(6) Readout of Concentration Factor. Press the “Mode” key to switch Factor Input Mode.
When the LCD shows “Input Factor: ”, the value is the current concentration factor. Use “Adj 0”
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
