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9.2.6
Method of Undetermined Coefficients
Click “Curve Type” in the menu bar. Select the type of curve that you need in the pull-down
sub-menu (The default setting is linear curve.).
Then click “Data” in the menu bar and select “Build Curve” in the sub-menu. A “Working
Curve Window” will appear on the screen and a working curve of the standard sample will be
plotted. Below the working curve
there are an equation of the working curve and correlation
coefficient.
The method as described above by which an equation of working curve is created through
measurement of standard samples is called Method of Undetermined Coefficients.
If an equation of working curve is known, you may select “Input Coef.”. As for the measuring
of samples by the method of inputting coefficient, refer to Section 9.1.3.
9.2.7
Measurement of Samples
After placing the sample into the sample compartment, click the “Measure Sample.” shortcut
on the bottom of the screen. The fluorescence value and the corresponding concentration value of
the sample will be displayed in the display box of sample measurement.
9.2.8
Set Sample Name
Click one grid of “Sample Name” in the “Unknown Sample” box to edit the name of selected
sample.
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
