P/N 53063-30, Manual revision: B
4-3
Preparing for a Cell Saver 5+ Procedure
Washing the red blood cells
After the process described above has been completed, the optical sensors
will detect that the RBC content of the bowl is sufficient to warrant washing
(at least 50% hematocrit).
The optical RBC sensor will initiate clamping the red-coded fill line and
opening the yellow-coded wash line. This causes saline solution to enter the
bowl and wash the red blood cells. Washing the RBCs removes unwanted
components such as cell stroma, free hemoglobin, activated clotting factors,
platelets, and AC solution.
Warning: Saline solution designated for intravenous use is the most suitable
solution for use with Cell Saver equipment.
At the end of the WASH state, just before the system enters the EMPTY or
RETURN state, the red valve will open for two pump revolutions, then close
while the blue valve opens. The two pump revolutions will force any saline
remaining in the line into the red-coded fill line, where it will be returned to
the bowl and eventually sent to the waste bag during the next fill cycle. If
this saline was left in the line, it would be sent to the reinfusion bag where it
would dilute the end product.
Washing partial bowls
In general, filling the bowl to a lower hematocrit will necessitate a higher
volume of wash solution to achieve adequate washout. Because the hemat-
ocrit of the bowl contents is lower, there is more supernatant in the bowl. In
order to dilute the larger volume of supernatant, two times the normal wash
solution (usually 2 x 1000 ml) is needed.
Emptying the bowl
Once the minimum wash volume of saline has been introduced and the
effluent line sensor has detected adequate washing, the wash line will be
clamped and the reinfusion line will be opened. The pump then reverses
direction, sending packed RBCs suspended in saline solution from the bowl
to the reinfusion bag.
The above cycle of FILL-WASH-EMPTY will be repeated as often as blood
loss requires. The reservoir level sensor will initiate additional cycles as
blood begins to be collected again and reaches the appropriate level to
initiate a fill cycle.
