P/N 53063-30, Manual revision: B
7-7
Sequestering using the Cell Saver 5+ System
Caution: Both clamps should never be closed at the same time.
"
Continue to observe the centrifuge bowl as it fills.
Plasma should flow into the PPP bag at a consistent flow rate until the white
buffy coat band (made up of platelets and white cells) which is immediately
adjacent to the top of the red cell layer reaches the shoulder of the bowl. As
the buffy coat exits the bowl:
"
Unclamp the blue-coded line to the PRP bag.
"
Clamp the clear line to the PPP bag.
When the effluent line flow from the bowl turns medium red (indicating the
presence of red blood cells):
"
Press the Empty key to stop both the centrifuge and the pump.
Emptying the bowl
At this point the operator can proceed to empty the bowl as follows:
"
Open the clamp on the waste bag line.
"
Close the clamp on the blue-coded line to the PRP bag.
Once the centrifuge has completely stopped, the pump will transfer the
RBCs to the reinfusion bag up to 200 ml/min and display the following infor-
mation:
Figure 7-6, Example of the EMPTY state screen display
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