P/N 53063-30, Manual revision: B
7-2
Sequestering using the Cell Saver 5+ System
P
RESENTING
THE
C
ELL
S
AVER
5+
SEQUESTERING
PROTOCOL
This chapter explains how to perform the sequestering protocol using the
CS5+ system. This protocol provides the possibility to collect
Platelet Rich
Plasma
(PRP) just after the induction of anesthesia but prior to the surgical
procedure.
The sequestering protocol is usually performed before the cell saving
protocol and is only available when the device is first powered on.
The CS5+ system should first be prepared with the standard processing set
(Latham bowl 225 or 125 ml).
Sequestering is not available with the 70mL Bowl set.
If sequestering of Platelet Rich Plasma is desired, the sequestering set LN244
should be attached to the standard bowl processing set as described in this
chapter or in the Directions For Use (DFU) provided with the set.
Describing method of sequestering
During the sequestering, the blood is collected into blood packs containing AC
solution. The blood is separated into platelet poor plasma (PPP), platelet rich
plasma (PRP) and red blood cells (RBCs). Typical processing time using this
method is 25 to 40 minutes. During this time approximately 1200 to 2500 ml
of whole blood is processed resulting in the collection of 1.5 to 3.0 x 10e11
platelets in 500 to 1600 ml of plasma.
*
Typically the pH is 7.02 at 6 hours and 7.01 at 24 hours post-collection when
stored in accordance with the current applicable standards.
*
Data on file at Haemonetics Corporation. Actual results may vary depending on patient
platelet pre-count, hematocrit, height, weight, physical condition, etc.
