PDA-100 Photodiode Array Detector
B-4
Doc. 031644-02 9/03
3. Connect a backpressure line made from 0.25-mm (0.010-in) ID
tubing to the cell outlet connection (see Figure B-2).
The backpressure line should provide enough restriction to generate
the small amount of backpressure (0.14 MPa to 0.34 MPa; 20 psi to
50 psi) required to help prevent bubbles from forming in the cell.
a. Locate the following items in the PDA-100 Ship Kit: 0.25-mm
(0.010-in) ID tubing (P/N 052306), two ferrule fittings
(P/N 043276), and two 10-32 bolts (P/N 043275).
b. Cut the 0.25-mm (0.010-in) ID tubing to the required length. The
backpressure line should be 1 meter long for a flow rate of
1.0 mL/min, 2 meters for 0.5 mL/min, and so on.
c. Install a ferrule fitting and 10-32 bolt on each end of the tubing.
d. Install the backpressure line after the cell outlet. Use a union
(P/N 042627) to connect the backpressure line to the waste line.
4. The cell connections exit the detector through slots in the front panel.
Thread the cell outlet tubing through the slot near the bottom of the
front panel; thread the cell inlet tubing through the slot on the right
side. Push the front panel into place.
5. Connect the cell outlet tubing to the separator column outlet.
Figure B-2. Flow Cell Connections
Operating at backpressures greater than 2 MPa (300 psi) or 3 MPa
(500 psi) will damage the PEEK or stainless steel cell heat exchanger,
respectively.
Cell Inlet Tubing
Cell Outlet Tubing
Heat Exchangers
Flow Cell
Handle
Spring Clip
