4 • Troubleshooting
Doc. 031644-02 9/03
4-9
4.8
Wavelength Verification Fails
•
Detector wavelength requires recalibration
In PeakNet 6 or Chromeleon, select the
UV Calibration
command on the
Wellness panel. Or, press F8 to display the Commands dialog box and select
UV
(to display UV-related commands). Select the
UV_Calibration
command, and then select
WavelengthCal
.
•
No solvent flow through cell
If the
Power
LED is flashing (see Figure 2-1), flush the cell with deionized
water or methanol and then turn off the detector power briefly. Turn on the
power again and verify that the
Power
LED is lighted, but is not flashing.
If solvent sits in the cell for an extended period, it decreases the intensity of
the light passing through the cell. To ensure reliable data, maintain a constant
flow through the cell. Flushing the cell with deionized water or methanol each
time the detector is powered up is helpful.
•
Lamp is dimming
Check the lamps as follows: In PeakNet 6 or Chromeleon, press F8 to display
the Commands dialog box and select
UV
(to display UV-related commands).
Select
UVLampAge
and then
VisLampAge
. If either lamp has been in
operation for more than 1000 hours, replace it. See Section 5.7 or Section 5.8
for instructions on replacing the deuterium lamp or tungsten lamp,
respectively.
•
Solvent absorbs at the wavelength reported for verification
Pump deionized water or methanol through the system during the wavelength
verification test.
