4 • Troubleshooting
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4-7
isocratic analyses in a laboratory with a stable ambient temperature. For more
information, see Section 3.4.
•
Absorbance in the reference wavelength
Sample absorbance in the selected reference wavelength may cause excessive
baseline drift. Select a reference bandwidth in an area of the spectrum where
little absorption occurs (see Section 3.4).
Dionex recommends selecting a reference wavelength (active reference
mode) for gradient analyses and turning off the reference wavelength for
isocratic analyses in a laboratory with a stable ambient temperature. For more
information, see Section 3.4.
4.5
Lamp Does Not Light or Lamp LED Is Flashing
•
Lamp is old or burned out
Replace the lamp (see Section 5.7 or Section 5.8 for instructions on replacing
the deuterium lamp or tungsten lamp, respectively). The average lamp
lifetime is 1000 hours, although lamps often last longer with only minimal
deterioration in performance.
4.6
Deuterium Lamp Intensity Count Too Low
If the deuterium lamp intensity reading is low (see Section 3.6), follow these steps
to resolve the problem.
•
Light signal is not reaching the data processor; reading is <1000 counts
Make sure that at least one lamp is turned on.
•
Low light throughput; reading is <10,000,000 counts
Remove the cell from the optical bench (see Section 5.4). Insert a business
card into the cell mounting location and check that bright light is shining onto
the card.
If you can see bright light shining onto the business card, remove the card
from the optical bench, run the lamp intensity calibration (see
Section 3.6), and then autozero the detector. If no error message is
displayed, flush the cell with eluent and/or clean the cell as instructed in
(see Section 5.2). Reinstall the cell in the optical bench and autozero the
