BD FACSymphony A1 Flow, User Manual

Page: 123 / 126

Index 123
fluidics 78
flow rate control buttons 17
fluid control buttons
PRIME 17
RUN 16
STANDBY 17
fluidics
description 16, 78
flow rate control 17
flushing system 40
preparing 32
removing air bubbles 29
run mode 16
sample injection port (SIP) 20
fluidics controls, cytometer 15
fluidics interconnect 30
fluorescence
defined 79
emission 84
fluorescence signal, none 92
fluorochromes
compensation theory 83
measured 35
role in light emission 79
flushing the system 40
forward scatter (FSC) 79
FSC See forward scatter 79
G
gating data 71
global worksheets
analyzing data 71
creating 68
previewing data 68, 75
H
hazard symbol definitions 8
Help, accessing 8
High Throughput Sampler (HTS) 12, 16, 20, 90
Horizon fluorochromes 101
hydrodynamic focusing 78
I
immunophenotyping
analysis 71
experiment 68
hydrodynamic focusing 78
L
lasers
performance check 55
quality control (QC) particles 118
wavelengths and power 22
longpass (LP) filters
defined 80
theory 81
LP See longpass filters 80
M
maintenance
Bal seal 46
cytometer shutdown 40
replacing waste tank cap 42
sample tube O-ring 48
schedule 38
sheath filter 43
system flush 40
mirrors, dichroic longpass (LP) filter 22
N
notch filters 80
O
O-ring
ordering 119
replacing 48
worn 90
online Help 8
optic holder 35
optics
components 22
configuration 53-54
dichroic mirrors 82