BD FACSymphony A1 Flow, User Manual

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5. Install a sample tube on the SIP to ensure that the outer sleeve has been properly installed.
If the sleeve hits the bottom of the tube, loosen the retainer slightly and push the sleeve up as far as it will
go. Tighten the retainer.
Changing the sample tube O-ring
Introduction
This topic describes how to replace the sample tube O-ring.
The sample tube O-ring, located within the retainer, forms a seal that allows the droplet containment vacuum
to function properly.
When to replace the O-ring
Replace the O-ring when droplets form at the end of the sample injection tube while the vacuum is operating.
Cytometer hardware might be contaminated with biohazardous material. Wear suitable protective
clothing, eyewear, and gloves whenever cleaning the cytometer or replacing parts.
Procedure
To change the O-ring:
1. Remove the outer sleeve from the sample injection tube by turning the retainer counter-clockwise.
2. Slide the outer sleeve from the retainer. See the following illustration.
2
1
Number Components
1 O-ring
2 Retainer
3. Invert the outer droplet sleeve and allow the O-ring to fall onto the benchtop.
If the O-ring does not fall out initially, hold the O-ring with your free hand and slide the outer sleeve to
remove the O-ring.
4. Place the new O-ring into the retainer. Make sure the O-ring is seated properly in the bottom of the retainer.
5. Replace the outer sleeve in the retainer.
48 BD FACSymphony™ A1 Flow Cytometer User's Guide