BD FACSymphony A1 Flow, User Manual

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3. Set the current tube pointer to Beads_001 .
4. Click Acquire Data in the Acquisition Dashboard to begin acquisition.
5. While data is being acquired:
a. Draw a gate around the singlets on the FSC vs SSC plot.
b. Rename the P1 gate to Singlets .
c. Use the Inspector to set the other plots to show only the singlet population by selecting the Singlets
checkbox.
6. Click Record Data.
7. When event recording has completed, remove the first tube from the cytometer.
The MyData worksheet plots should look like the following.
8. Install the second sample tube onto the SIP.
9. Set the current tube pointer to Beads_002 .
10. Click Acquire Data to begin acquisition.
11. Before recording, preview the data on the MyData worksheet to verify that all expected populations are
visible and the data is similar to the previous sample.
12. Click Record Data.
13. When event recording has completed, remove the second tube from the cytometer.
14. If you are recording more than two tubes, repeat steps 8 through 13 for the remaining tubes.
70 BD FACSymphony™ A1 Flow Cytometer User's Guide