BD FACSymphony A1 Flow, User Manual

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3. Expand the Global Worksheets folder in MyExperiment to access the default global worksheet, and rename
the worksheet MyData .
4. On the MyData worksheet, create the following plots for previewing the data:
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FSC vs SSC
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FITC vs PE
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FITC vs PerCP-Cy5.5
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FITC vs APC
Applying saved application settings to a new experiment
When applications settings are applied to an experiment, the cytometer settings are updated with the
parameters included in the application settings, optimized PMT voltages, threshold settings, area scaling
factors, and window extension values.
To apply saved application settings to your experiment:
1. Right-click the experiment-level Cytometer Settings and select Application Settings > Apply.
2. In the Application Settings catalog, select the application settings file you saved previously and click Apply.
If the parameters are not the same, a mismatch dialog opens.
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Click Overwrite to update all settings.
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Click Apply to change only the common parameters.
For more information, see the BD FACSDiva™ Software Reference Manual .
The cytometer settings are renamed application settings and the cytometer settings icon in the Browser
changes.
More information
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Creating application settings (page 62)
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Recording data (page 69)
Recording data
Introduction
This topic provides an example of how to preview and record data for multiple samples.
Before you begin
Prepare the sample tubes.
Note: If you need to run samples at an event rate greater than 10,000 events/second, consider changing your
Window extension. See the BD FACSDiva™ Software Reference Manual for more information.
Recording data
To record data:
1. Press RUN and HIGH on the cytometer fluid control panel.
2. Install the first sample tube onto the SIP.
Chapter 6 Recording and analyzing data 69