BD FACSymphony A1 Flow, User Manual

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2. If bubbles are visible, gently tap the filter body with your fingers to dislodge the bubbles and force them to
the top.
When removing air bubbles, do not vigorously shake, bend, or rattle the sheath filter or you
might damage it.
3. Direct the vent line into a beaker and press the small button at the end of the vent fitting against the side of
the beaker until a steady stream of fluid empties from the filter.
2
1
Number Components
1 Button
2 Vent fitting
4. Tilt the filter and verify that no trapped air remains in the filter.
5. Repeat steps 3 and 4 until no air is observed in the filter.
6. Check the sheath line for air bubbles.
7. Open the roller clamp at the fluidics interconnect (if necessary) to bleed off any air in the line. Collect any
excess fluid in a waste container.
Note: The roller clamp can be found close to the fluidics ports of the cytometer.
8. Close the roller clamp.
Preparing the waste tank
Introduction
This topic describes how to prepare the waste tank. Prevent waste overflow by emptying the waste tank daily or
whenever the system status indicator turns yellow.
Note: If your system is connected to the FFSS, see the BD FACSFlow™ Supply System User’s Guide for more
information.
All biological specimens and materials coming into contact with them are considered biohazardous.
Handle as if capable of transmitting disease. Dispose of waste using proper precautions and in
accordance with local regulations. Never pipette by mouth. Wear suitable protective clothing,
eyewear, and gloves.
30 BD FACSymphony™ A1 Flow Cytometer User's Guide