5 Commissioning and First Operating Steps | 5.8 Working with Optional Accessories
ZEISS
9. Attach the specimen holder to the airlock rod by turning the knob clockwise.
5.8.1.2 Loading the Specimen Using an Airlock
You can load the specimen either manually or with pre-defined airlock macros to simplify the
specimen exchange.
Info
If you want to use a navigation camera at the airlock to navigate the stage, you have to ac-
quire an overview image before the sample holder is introduced into the specimen chamber.
This procedure consists of the following steps:
§
Loading the Specimen Holder into the Airlock [
§
Closing the gate valve and opening the column chamber valve
Controlling the Valves via Airlock Macros [
5.8.1.2.1 Loading the Specimen Holder into the Airlock
WARNING
Suffocation hazard due to lack of oxygen
Gaseous dry nitrogen is used to vent the specimen chamber during specimen exchange. Inhal-
ing nitrogen may cause unconsciousness.
4
During specimen exchange, keep the chamber door open as short as possible.
4
Do not inhale the air from within the specimen chamber.
4
Ensure that the area around the microscope is sufficiently vented.
4
If you begin to experience symptoms of asphyxia (for example: rapid breathing, loss of
mental alertness and/or muscular coordination, depression of sensations, emotional insta-
bility, fatigue) leave the room immediately and inform the facility’s safety officer.
Prerequisite
ü
The airlock is vented
ü
The correct specimen holder is mounted and is retracted into the airlock
ü
The specimen chamber is unloaded
Procedure
1. Insert the stub with the new specimen into the specimen holder and fix the stub to the
specimen holder.
2. Check that the airlock rod is fully retracted.
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Instruction Manual ZEISS SIGMA series | en-US | Rev. 7 | 352102-9344-006