Thermo Scientific TSQ 8000, Руководство пользователя

Страница: 92 / 150

4
Creating a Method
Creating a MS Instrument Method
70 TSQ 8000 Evo User Guide Thermo Scientific
Settings: Optimize is checked by default. When Optimize is checked, you can
adjust the settings in the pane to optimize your method. See Figure 68 on
page 69 . Remove the check from the box if you want to manually input your
acquisition windows. The default values for the window optimizer should give
reasonable results for normal methods. The default values are:
Desired Min Dwell Time—10 ms
Desired Window—0.6 min
Minimum Window—0.3 min
– Change the minimum dwell time using the Desired Min Dwell Time
combo box. If your method has many transitions, you may want to reduce
the desired minimum dwell time. Note that the wider the acquisition
windows in your method, the shorter the average dwell time will be.
– Change the desired acquisition window in the Desired Window combo box.
The desired window is the amount of time to scan for a transition around a
given retention time to ensure that compound will be observed. The desired
window can be set from 0.24–5 min. Set the window wide enough so that a
retention time shift will not cause you to miss any compounds. Include extra
time in this window if there is any uncertainty in compound retention times
in the method. Note that the longer the dwell time for your compounds, the
narrower your acquisition windows will be.
– Change the minimum acquisition window in the Minimum Window
combo box. The minimum window can be set from 0.24–5 min. This is the
smallest amount of time that should be scanned for a transition around a
given retention time so that you are confident the compound will be
observed. Set the minimum window to the lowest safe value to prevent
compound retention times from drifting outside the acquisition window.
ii. The Time Summary section reports the resulting total scan time, the SRM/SIM
time, and lowest dwell time for you method. These values are for information
only and not editable.
– The Resulting Total Scan Time is the baseline peak width divided by the
number of points desired across the peak. These values should be updated if
your method requirements are different from the defaults.
– The SIM/SRM time is the total length of all SIM or SRM scans for each
compound in your list. This will match the total scan time unless the
method also has a full scan event.
Note If the dwell time limit is reached and the minimum acceptable window
is forced below the 0.24 min limit, the method will fail, and a smaller list
must be used.