Thermo Scientific TSQ 8000, Руководство пользователя

Страница: 87 / 150

4
Creating a Method
Creating a MS Instrument Method
Thermo Scientific TSQ 8000 Evo User Guide 65
i. In the Name column, enter the analyte name. If you have linked to an external file,
the analyte name located in the first column of your linked file will appear in the first
cell in the Name column. Right-click this window to search for an analyte within
your method. See
Figure 64 . This function is useful if you need to edit an analyte in a
complex method.
Figure 64. Finding an Analyte in a Method
j. In the RT column, enter retention times for SRM methods. The retention time is the
time it takes an analyte to pass from the column inlet to the detector.
k. In the Window (min) column, set the acquisition times. Smaller acquisition
windows increase sensitivity but can cause you to miss your peak if set too small.
Changing the window size only affects sensitivity if it reduces the number of
compounds analyzed in a segment. If the windows do not overlap, you will not notice
an improvement by reducing the acquisition window.
l. In the Mass column, enter the precursor ion mass for SRM methods. For SIM
methods, enter the mass of the ion you wish to monitor.
m. In the Product Mass column, enter the product ion mass (for SRM methods). The
product ion mass is the result of fragmenting the precursor ion with collision gas.
n. In the Collision Energy column, enter the collision energy (for SRM methods) in
eV. The collision of a precursor ion with the collision gas at a particular energy
fragments the precursor ions into product ions. By varying the collision energy, a
single precursor ion can be fragmented into several product ions. An SRM transition
is defined by a precursor ion being fragmented into a particular product ion mass
using a certain collision energy.
Note The Window (min) column does not appear by default. The Method
Editor displays it only when the window optimizer is not active.
Right-click the
Name Column
to Find an
Analyte in a
Method