Thermo Scientific TSQ 8000, Руководство пользователя

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2
Changing the Column
Replacing the Factory Installed Column
20 TSQ 8000 Evo User Guide Thermo Scientific
d. Select Column and input the column’s physical characteristics.
e. If a pre-/post column is present, set the length and nominal internal diameter of the
pre-/post column in the same valid ranges for the column. The following two lines
are added to the menu.
f. Start the column evaluation. According to the physical characteristics of the column,
the system calculates and displays the relevant column K-factor. At the end of the
routine, a message will indicate that the evaluation was successful.
g. Expect a K-factor of approximately 0.7 – 0.9 for a 15 m, 0.25 mm i.d. column
(1.3 – 2.0 for a 30 m, 0.25 mm i.d. column). If the column does not report a
K-factor within this range or within 0.1 units of the previous stored value, check for a
leak or broken column using the leak detector. The K-factor is a measured resistance
for the column. A K-factor that is too low may indicate a leak in the system, while a
K-factor that is too high may indicate a blockage.
Fix any issues found and rerun column evaluation until an appropriate K-factor is
achieved.e a leak in the system, while a K-factor that is too high might indicate a
blockage.
11. Disconnect the column flowmeter:
a. Disconnect the column from the column flowmeter connector.
b. Remove the clear plastic component, including its fittings, from the oven and set
them aside.
c. Close the GC door.
12. Condition the column before inserting it into the TSQ 8000 system. Column
conditioning consists of passing a carrier gas flow through the column heated to a
programmed temperature as described in the column manufacturer’s instructions.
a. If there are no conditioning instructions, perform the column conditioning by setting
a final temperature 10 °C–20 °C below the column’s recommended maximum
temperature.
Note For the most reproducible results, you should conduct a more detailed
column evaluation. However, the following steps, while recommended, are not
required.
CAUTION INSTRUMENT DAMAGE: The material released from the column
(column bleed) during conditioning may contaminate the ion source if the
column is inserted into the transfer line during the high-temperature stage of
conditioning.
WARNING FIRE HAZARD: Do not use hydrogen as the carrier gas for
conditioning your column. It could vent into the oven and present an explosion
hazard.