Thermo Scientific TSQ 8000, Руководство пользователя

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4
Creating a Method
Creating a MS Instrument Method
56 TSQ 8000 Evo User Guide Thermo Scientific
All of these scans use the most recent AutoTune_EI file. You can also use a
specific tune file for each of the scans.
ii. Use the SRM, SIM, or Scan Masses column to tell the MS which masses to
scan.
– In full-scan mode, enter the start and end mass separated by a dash. You
must put each full-scan range on a separate line.
– For SRM methods, enter precursor masses, product masses, and collision
energies of the ions you wish to monitor. Separate scan filters will be created
for each SRM transition during acquisition.
– If you would like to combine multiple SRM transitions in a single scan filter,
highlight the rows you want to combine and then right-click and select
Group SRM Scans | Replace Selected Scans
. See Figure 55 . You can also use
this function to ungroup a group of SRM scans into individual rows.
Figure 55. Grouping SRM Scans
iii. Enter the product ion mass (for SRM methods) in the Product Mass column.
The product ion mass is the result of fragmenting the precursor ion with
collision gas. If you want to perform a precursor ion scan, enter the mass of the
target precursor ion into this column.
Note Each line in a scan must only contain a full-scan range, individual SIM
masses, or an SRM transition. Scan types cannot be mixed in a single line. Set
up simultaneous scans by giving them the same time in the
Time (min)
column.
Note
Entering a product ion mass changes a scan to SRM.