Thermo Scientific Applied Biosystems QuantStudio, Руководство пользователя

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About the chip
The QuantStudio
™
3D Digital PCR 20K Chip v2 is a 10-mm
2
high-density reaction
plate that has a single array of 20,000 reaction wells. Hydrophobic coatings on the
chip surface enable loading and isolation of independent PCR reactions within the
wells.
Once loaded with a reaction, the chip must be sealed using the QuantStudio
™
3D
Digital PCR Chip Lid v2 and filled with Immersion Fluid. Figure 2 shows the
components of the chip assembly.
443MP
J
443MPJ
1
2
3
5
443MPJ
4
7
6
Figure 2 QuantStudio
™
3D Digital PCR 20K Chip v2
1
QuantStudio ™ 3D Digital PCR Chip Lid v2
—The lid used to seal the chip for thermal cycling
and imaging.
2
QuantStudio
™
3D Digital PCR 20K Chip v2 —A 10-mm
2
chip with 20,000 wells that contain
the individual PCR reactions.
3
Chip case —The thermal-conductive base that secures and protects the chip.
4
Chip ID
—A unique alphanumeric ID on the chip lid.
5
Fill port —The hole in the chip case lid through which Immersion Fluid is injected onto the
chip.
6
Reaction wells —The 20,000 physical holes on the surface of the chip that contain the
individual PCR reactions.
7
2D barcode —A barcode that encodes the same alphanumeric string as the Chip ID, scanned
by the QuantStudio
™
3D Digital PCR Instrument for chip tracking.
Chapter 1 Product information
About the chip
1
14
QuantStudio ™ 3D Digital PCR System User Guide