Thermo Scientific Applied Biosystems QuantStudio, Руководство пользователя

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3.
Carefully dispense Immersion Fluid into the port until the chip case contains an
air bubble slightly larger than the fill port (<2–3 mm in diameter). Rotate the chip
slightly to expose any hidden bubble. If a bubble larger than 2–3 mm is present,
add additional fluid.
4.
Using a low-lint wipe, remove any excess Immersion Fluid from the chip case to
ensure optimal imaging.
Proceed to sealing the chip case.
Seal the chip case with the label
1.
Gently pull back the top half of the label on the chip lid.
2.
Remove the label backing and press the label firmly over the fill port for
5 seconds to ensure a tight seal.
443MPJ
443MPJ
IJ
IMPORTANT!
Press only on the label region of the chip lid. Pressure placed on
the window of the chip lid can expel the PCR reactions out of the chip wells.
3.
Gently run your fingers over the entire label to seal the remainder of the label.
4.
Inspect the sealed chip for potential problems:
•
Leaks – Confirm that no fluid is leaking from the fill port or from the seal
between the chip and lid.
• Bubbles – Check for large or multiple bubbles inside the chip case. One
small air bubble is acceptable.
• Correct lid orientation
– Confirm that the lid is correctly aligned on the
chip.
If the sealed chip fails any of the criteria above, discard it and prepare another
chip. The sealed chip cannot be opened and resealed.
5.
Store the prepared chip in a clean, dry, dark location until you are ready to load
it onto the thermal cycler.
Thermal cycle prepared chips within 2 hours after loading them.
Note: You can label the back of the chip with a permanent marker. This will not
affect the imaging data.
Appendix E Load chips manually
Seal the chip case with the label
E
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QuantStudio ™ 3D Digital PCR System User Guide