PerkinElmer TORION T-9, Руководство пользователя

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180 . Torion T-9 User’s Guide
Deconvolution Parameters Tab (Performance Validation)
There are two Deconvolution Parameters in Chromion software, one in Target Library Editor and
the other one in Performance Validation . They have similar functions but are used for different
purposes.
The Deconvolution Parameters tab in Target Library Editor is used for deconvolution of samples
while the Deconvolution Parameters tab in Performance Validation is used to test the
performance of the instrument when a specific standard is being analyzed. Because the settings for
both of these are independent of one another they are stored in separate locations.
The settings on the
Deconvolution Parameters
tab is only accessible by clicking the unlock icon
and entering the password. See the icon description for Unlock Performance Validation Tests
on the mini toolbar at the beginning of this section for instructions on how to use the unlock feature.
NOTE: For details on how to set the parameters refer to the Deconvolution Parameters tab in the
Target Library Editor section found earlier in this chapter. To ensure that the performance
validation works correctly it is strongly suggested that the default deconvolution settings be used
unless there has been adequate training on how to successfully make changes to this section. For
more information contact PerkinElmer.
Performance Validation Settings Tab
The only setting in the
Performance Validation Settings
tab is the
End early time
. The last
peak of the standard used to run a performance validation elutes from the column at 85 seconds. A
normal analysis runs to a higher temperature and takes
about 130 seconds. This setting can be adjusted to
shorten the time it takes to run a performance
validation. This value must be manually entered. The
default value is 100. When the value is set to 0 the
performance validation will use the run time that is set in the
Gas Chromatograph
screen. To set
this parameter it is necessary to unlock the
Performance Validation
form. See the icon
description for Unlock Performance Validation Tests in the mini toolbar at the beginning of this
section for instructions on how to use the unlock feature.
Mass Range Tab
The Torion T-9 has the ability to set a mass range. Ion trap mass spectrometers use algorithms to
control the ionization time and to determine response. These algorithms have improved performance
when there are more data points to work with. The instruments full range of
43-500 amu
requires a
scan time of 60 milliseconds. The Torion T-9 has a
fast high resolution gas chromatograph. This means
that the peaks are narrow which results in fewer
scans per peak. With a 60 ms scan time there will
be approximately 5-7 scans across a peak. Setting
the mass range to 43-320 amu changes the scan
time to 36 ms resulting in about twice as many
scans across a peak. If the analytes being analyzed are not expected to have masses greater than 320
amu then it is best to use the lower range which will improve the algorithms control over the mass
analyzer. The other mass ranges are available in cases where higher molecular weight analytes are
expected.
Most analytes that can be eluted through the Torion T-9 GC-MS instrument will have masses that are
less than 320 amu. The following table shows the scan times for the three available mass ranges.
Mass range (amu) Scan Time (ms)
43 - 320 36
43 - 400 45
43 - 500 60